Taura T, Giblin F J, Reddy V N
Exp Eye Res. 1985 Oct;41(4):527-43. doi: 10.1016/s0014-4835(85)80010-5.
The effect of a 30% galactose diet on the progression of X-ray-induced cataract in mice was evaluated by following morphological changes as seen by light and transmission electron microscopy in different regions of the lens. Lens opacities as observed with the slit-lamp biomicroscope developed at a slower rate in galactose-fed animals than in those on a normal diet. The protective effect of galactose on X-ray cataract was seen whether galactose feeding was initiated either 1 week before or after exposure to X-ray. At 4 months after X-ray approximately 50% of galactose-fed animals had mature cataracts, compared to 100% in the control group. Similarly, at two weeks after exposure to X-ray, before any lens opacities were observed, morphological changes were more severe in the control group; cells in the meridional row were more disorganized in the control than in the galactose-fed groups. However, the progression of mature cataracts in the two galactose-fed groups were not significantly different. Since free radicals produced by X-ray are thought to be short-lived, the protective effect of galactose feeding after X-ray was unexpected, raising the possibility that some of the active species may be long-lasting. The nature of such radicals, if any, is unknown and remains to be investigated.
通过光学显微镜和透射电子显微镜观察晶状体不同区域的形态变化，评估30%半乳糖饮食对X射线诱导的小鼠白内障进展的影响。用裂隙灯生物显微镜观察到，半乳糖喂养的动物晶状体混浊的发展速度比正常饮食的动物慢。无论在X射线照射前1周还是照射后开始喂养半乳糖，都能观察到半乳糖对X射线白内障的保护作用。在X射线照射后4个月，约50%的半乳糖喂养动物出现成熟白内障，而对照组为100%。同样，在X射线照射后两周，在观察到任何晶状体混浊之前，对照组的形态学变化更严重；对照组子午线排的细胞比半乳糖喂养组更紊乱。然而，两个半乳糖喂养组中成熟白内障的进展没有显著差异。由于X射线产生的自由基被认为是短命的，X射线照射后喂养半乳糖的保护作用出乎意料，这增加了某些活性物质可能持久存在的可能性。此类自由基（如果存在）的性质尚不清楚，有待进一步研究。
