Wu Liwen, Cai Yuqing, Jiang Chenggang, Shi Xiang, Xiong Shifa, Chen Yicun, Wang Yangdong
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, No. 1 Dongxiaofu, Xiangshan Road, Beijing Haidian District, Beijing 100091, China.
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, No. 73 Daqiao Road, Hangzhou Fuyang District, Zhejiang 311400, China.
Hortic Res. 2025 Jul 9;12(9):uhaf156. doi: 10.1093/hr/uhaf156. eCollection 2025 Sep.
is a common timber oak tree species widely distributed in subtropical areas of China. In this study, we presented a chromosome-scale reference genome assembly of . achieved by integrating PacBio Sequel II, DNBseq™, and Hi-C sequencing platforms, and the results indicated the . genome has a size of 836.74 Mb. Through the analysis of significantly expanded gene families, we identified that many of the top-ranked KEGG pathways are associated with amino acid metabolism. Subsequently, we performed an amino acid metabolic profile analysis on . and related species, including , , and . The findings revealed that the content of amino acids in . was significantly higher than that in the other three oak species. Additionally, we found a significantly higher content of flavor amino acids, such as glutamic acid (Glu), aspartic acid (Asp), and glycine (Gly), in . . Considering these results, we designed experiments to assess the nutrient content in mushrooms cultivated from the four oak trees. The results indicated that the total amino acid and protein content of mushrooms cultivated using . as a substrate was significantly greater than that of mushrooms grown on the other three oak species. This characteristic may explain why . wood is particularly effective as a substrate for cultivating more flavorful mushrooms. This study presents the complete genome and evolutionary information of , and integrates metabolic profiling to explore the underlying reasons for the enhanced flavor of mushrooms cultivated from it.
是一种常见的用材栎树树种，广泛分布于中国亚热带地区。在本研究中，我们展示了通过整合PacBio Sequel II、DNBseq™和Hi-C测序平台获得的……的染色体水平参考基因组组装，结果表明……基因组大小为836.74 Mb。通过对显著扩张的基因家族进行分析，我们确定许多排名靠前的KEGG通路与氨基酸代谢相关。随后，我们对……以及相关物种，包括……、……和……进行了氨基酸代谢谱分析。研究结果显示，……中的氨基酸含量显著高于其他三种栎树物种。此外，我们发现……中风味氨基酸如谷氨酸（Glu）、天冬氨酸（Asp）和甘氨酸（Gly）的含量显著更高。考虑到这些结果，我们设计实验评估了用这四种栎树培育的蘑菇中的营养成分。结果表明，以……为底物培育的蘑菇的总氨基酸和蛋白质含量显著高于在其他三种栎树上生长的蘑菇。这一特性可能解释了为什么……木材作为培育风味更佳蘑菇的底物特别有效。本研究展示了……的完整基因组和进化信息，并整合代谢谱分析来探究用其培育的蘑菇风味增强的潜在原因。
