Sabooni Sallama, Salah Husam, Singh Rajvir, Al-Qadi Khloud, Taj-Aldeen Saad J, Ibrahim Emad
Division of Microbiology, Department of Laboratory Medicine & Pathology, Hamad Medical Corporation, Doha, Qatar.
Heart hospital, Hamad Medical Corporation, Doha, Qatar.
Qatar Med J. 2025 Aug 20;2025(3):78. doi: 10.5339/qmj.2025.78. eCollection 2025.
is a common pathogenic parasite known to cause diarrhea in humans and animals, particularly in young children living in poor conditions. Although diarrheal disease is usually mild in immunocompetent individuals, it may progress into a life-threatening complication among the immunocompromised. Due to insensitive conventional diagnostic methods, the identification of can be inaccurate and challenging. The present study aimed to investigate the prevalence of cryptosporidiosis infection in Qatar by comparing four different diagnostic methods for detecting in human stool samples.
From January 2018 to December 2019, stool samples from patients with various gastrointestinal symptoms were collected at the microbiology laboratory at Hamad General Hospital in Qatar for parasitic detection. The stool samples were tested using four diagnostic methods: routine microscopy, immunochromatography (ICT), multiplex polymerase chain reaction (PCR), and modified Kinyoun's acid-fast stain (MKS).
In the evaluation of the four different detection methods, 36 (18%) out of 205 stool samples tested positive for , with detection rates of 18%, 15%, 7%, and 6% using PCR, ICT, MKS, and routine microscopy, respectively.
The superior sensitivity of PCR and ICT supports their integration into routine diagnostics to improve the detection and public health surveillance of cryptosporidiosis in Qatar.
是一种常见的致病性寄生虫，已知可导致人类和动物腹泻，尤其是生活在贫困条件下的幼儿。虽然腹泻病在免疫功能正常的个体中通常较轻，但在免疫功能低下者中可能进展为危及生命的并发症。由于传统诊断方法不敏感，对的鉴定可能不准确且具有挑战性。本研究旨在通过比较四种不同的诊断方法检测人类粪便样本中的，来调查卡塔尔隐孢子虫病感染的流行情况。
2018年1月至2019年12月，在卡塔尔哈马德总医院微生物实验室收集有各种胃肠道症状患者的粪便样本进行寄生虫检测。粪便样本使用四种诊断方法进行检测：常规显微镜检查、免疫层析法（ICT）、多重聚合酶链反应（PCR）和改良金胺酚染色法（MKS）。
在对四种不同检测方法的评估中，205份粪便样本中有36份（18%）检测呈阳性，使用PCR、ICT、MKS和常规显微镜检查的检出率分别为18%、15%、7%和6%。
PCR和ICT的卓越敏感性支持将它们纳入常规诊断，以改善卡塔尔隐孢子虫病的检测和公共卫生监测。
