Palacín-García R, Goñi L, Río T Gómez-Del
Durability and Mechanical Integrity of Structural Materials, Rey Juan Carlos University, Tulipán s/n, 28933 Madrid, Spain.
Biomimetics (Basel). 2025 Jul 24;10(8):491. doi: 10.3390/biomimetics10080491.
The development of alginate/polyacrylamide hydrogels for various biomedical applications has attracted significant interest, particularly due to their potential use in wound healing and tissue engineering. This study explores the fabrication of these hydrogels via 3D bioprinting with ultraviolet light curing, focusing on how the alginate concentration and curing speed impact their mechanical properties. Rheological testing was employed to examine the viscoelastic behavior of alginate/polyacrylamide hydrogels manufactured using a 3D bioprinting technique. The relaxation behavior and dynamic response of these hydrogels were analyzed under torsional stress, with relaxation curves fitted using a two-term Prony series. Fourier Transform Infrared (FTIR) spectroscopy was also employed to assess biocompatibility and the conversion of acrylamide. This study successfully demonstrated the printability of alginate/polyacrylamide hydrogels with varying alginate contents. The rheological results indicated that 3D bioprinted hydrogels exhibited significantly high stiffness, viscoelasticity, and long relaxation times. The curing speed had a minimal impact on these properties. Additionally, the FTIR analysis confirmed the complete conversion of polyacrylamide, ensuring no harmful effects in biological applications. The study concludes that 3D bioprinting significantly enhances the mechanical properties of alginate/polyacrylamide hydrogels, with the alginate concentration playing a key role in the shear modulus. These hydrogels show promising potential for biocompatible applications such as wound healing dressings.
用于各种生物医学应用的藻酸盐/聚丙烯酰胺水凝胶的开发引起了广泛关注，特别是由于它们在伤口愈合和组织工程中的潜在用途。本研究探索了通过紫外光固化3D生物打印制备这些水凝胶，重点研究藻酸盐浓度和固化速度如何影响其机械性能。采用流变学测试来研究使用3D生物打印技术制造的藻酸盐/聚丙烯酰胺水凝胶的粘弹性行为。在扭转应力下分析这些水凝胶的松弛行为和动态响应，并用双项Prony级数拟合松弛曲线。还采用傅里叶变换红外（FTIR）光谱来评估生物相容性和丙烯酰胺的转化率。本研究成功证明了不同藻酸盐含量的藻酸盐/聚丙烯酰胺水凝胶的可打印性。流变学结果表明，3D生物打印水凝胶表现出显著高的刚度、粘弹性和长松弛时间。固化速度对这些性能影响最小。此外，FTIR分析证实了聚丙烯酰胺的完全转化，确保在生物应用中无有害影响。该研究得出结论，3D生物打印显著提高了藻酸盐/聚丙烯酰胺水凝胶的机械性能，藻酸盐浓度在剪切模量中起关键作用。这些水凝胶在伤口愈合敷料等生物相容性应用中显示出有前景的潜力。
