Kižys Kasparas, Pirštelis Domas, Bružaitė Ingrida, Morkvėnaitė Inga
Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology, Sauletekio 3, LT-10257 Vilnius, Lithuania.
Biosensors (Basel). 2025 Aug 9;15(8):519. doi: 10.3390/bios15080519.
Microbial fuel cells (MFCs) are one of the contributors to the novel sustainable energy generation from organic waste. However, the application of MFCs is limited due to the slow charge transfer between cells and electrodes. This problem can be solved by modifying cells with conductive polymers, such as polypyrrole (PPy). We investigated the viability and electroactivity of modified cells at five different pyrrole concentrations, namely 8, 25, 50, 100, and 200 mM. The 100 mM concentration of PPy solution had the highest impact on yeast cells' proliferation and growth, with the CFU/mL of PPy-treated yeast cells being 0.6 × 10 ± 5 × 10. The power density of the constructed MFC was evaluated by using an external load. The MFCs were analyzed using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Although CV results with different pyrrole concentrations were similar, DPV indicated that yeast modification with 50 mM pyrrole resulted in the most significant current density, which may be attributed to an increase in charge transfer due to the conductive properties of polypyrrole. The power density achieved with modified yeast in wastewater, 12 mW/m, reached levels similar to those in laboratory solutions, 45 mW/m.
微生物燃料电池（MFCs）是利用有机废物产生新型可持续能源的方式之一。然而，由于电池与电极之间的电荷转移缓慢，MFCs的应用受到限制。这个问题可以通过用导电聚合物（如聚吡咯（PPy））修饰电池来解决。我们研究了在五种不同吡咯浓度（即8、25、50、100和200 mM）下修饰电池的活力和电活性。100 mM浓度的PPy溶液对酵母细胞的增殖和生长影响最大，经PPy处理的酵母细胞的CFU/mL为0.6×10±5×10。通过使用外部负载评估构建的MFC的功率密度。使用循环伏安法（CV）和差分脉冲伏安法（DPV）对MFCs进行分析。尽管不同吡咯浓度下的CV结果相似，但DPV表明用50 mM吡咯修饰酵母产生的电流密度最为显著，这可能归因于聚吡咯的导电特性导致电荷转移增加。在废水中用修饰酵母实现的功率密度为12 mW/m²，达到了与实验室溶液中相似的水平，即45 mW/m²。
