Song Chenxia, Wang Yan, Sun Tao, Han Yi, Mu Yanjuan, Ji Xinyue, Zhang Shuxin, Sun Yanguo, Wu Fusheng, Liu Tao, Li Ningning, Han Qingjun, Tong Boqiang, Lu Xinghui, Lu Yizeng
College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China.
Institute of Germplasm Innovation, Shandong Provincial Center of Forest and Grass Germplasm Resources, Key Laboratory of National Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources, Jinan 250102, China.
Metabolites. 2025 Jul 22;15(8):493. doi: 10.3390/metabo15080493.
: 'Jinsi' has excellent wood properties and golden texture, which is widely used in producing furniture and crafts. The lignin content and structural composition often determine the use and value of wood. Hence, investigating the characteristics of the annual dynamics of lignin anabolic metabolites in 'Jinsi' and analyzing their synthesis pathways are particularly important. : We carried out targeted metabolomics analysis of lignin synthesis metabolites using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) on the xylem samples of 'Jinsi' in February, April, July, October 2022, and January 2023. : A total of 10 lignin synthesis-related metabolites were detected: L-phenylalanine, cinnamic acid, -coumaraldehyde, sinapic acid, -coumaric acid, coniferaldehyde, ferulic acid, sinapaldehyde, caffeic acid, and sinapyl alcohol (annual total content from high to low). These metabolites were mainly annotated to the synthesis of secondary metabolites and phenylpropane biosynthesis. The annual total content of the 10 metabolites showed the tendency of "decreasing, then increasing, and then decreasing". : 'Jinsi' is a typical G/S-lignin tree species, and the synthesis of G-lignin occurs earlier than that of S-lignin. The total metabolite content decreased rapidly, and the lignin anabolism process was active from April to July; the metabolites were accumulated, and the lignin anabolism process slowed down from July to October; the total metabolite content remained basically unchanged, and lignin synthesis slowed down or stagnated from October to January of the following year. This reveals the annual dynamic pattern of lignin biosynthesis, which contributes to improving the wood quality and yield of 'Jinsi' and provides a theoretical basis for its targeted breeding.
金丝檀木具有优良的木材特性和金色纹理，广泛用于生产家具和工艺品。木质素含量和结构组成往往决定木材的用途和价值。因此，研究金丝檀木中木质素合成代谢物的年度动态特征并分析其合成途径尤为重要。我们使用超高效液相色谱-串联质谱法（UPLC-MS/MS）对2022年2月、4月、7月、10月以及2023年1月的金丝檀木木质部样本进行了木质素合成代谢物的靶向代谢组学分析。共检测到10种与木质素合成相关的代谢物：L-苯丙氨酸、肉桂酸、对香豆醛、芥子酸、对香豆酸、松柏醛、阿魏酸、芥子醛、咖啡酸和芥子醇（年度总含量从高到低）。这些代谢物主要注释到次生代谢物合成和苯丙烷生物合成。这10种代谢物的年度总含量呈现出“先下降，再上升，然后再下降”的趋势。金丝檀木是典型的G/S-木质素树种，G-木质素的合成早于S-木质素。4月至7月，总代谢物含量迅速下降，木质素合成代谢过程活跃；7月至10月，代谢物积累，木质素合成代谢过程放缓；次年10月至1月，总代谢物含量基本保持不变，木质素合成放缓或停滞。这揭示了木质素生物合成的年度动态模式，有助于提高金丝檀木的木材质量和产量，并为其定向育种提供理论依据。
