Gao Yu, Huang Siyuan, Zhang Jingling, Zhu Lin, Zhan Baocan, Yu Xiaohui, Chen Yinhua
State Key Laboratory of Tropical Crop Breeding, Sanya Institute of Breeding and Multiplication, Hainan University, Sanya 570025, China.
School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
J Fungi (Basel). 2025 Aug 19;11(8):601. doi: 10.3390/jof11080601.
Mutualism between plants and arbuscular mycorrhizal fungi (AMF) is imperative for sustainable agricultural production. Jasmonic acid (JA) signal transduction has been demonstrated to play an important role in AMF symbiosis with the host. In this study, SC9 cassava was selected as the research object to investigate the effect of the jasmonic acid signaling pathway on symbiosis establishment and cassava growth in AMF and cassava symbiosis. It was first found that the symbiosis of cassava and mycorrhizal fungi could increase the biomass of both the aboveground and belowground parts of cassava. Secondly, JA content increased significantly in the early stage of AMF inoculation and auxin content increased significantly in the late stage of AMF inoculation, suggesting that JA signal transduction played an important role in the symbiosis between cassava and mycorrhizal fungi. Transcriptome data were used to analyze the expression differences of genes related to JA synthesis and signal transduction in cassava. The gene positively responded to symbiosis between cassava and mycorrhizal fungi. The analysis of MeJAZ gene family expression and its promoter supported this result. Spraying different concentrations of MeJA on leaves could affect the colonization rate and root biomass of cassava, indicating that JA was an active regulator of mycorrhizal formation. PPI prediction and qPCR analysis suggested that the gene might be a key transcriptional regulator responding to jasmonic acid signals and regulating mycorrhizal influence on cassava growth and development.
植物与丛枝菌根真菌（AMF）之间的共生关系对可持续农业生产至关重要。茉莉酸（JA）信号转导已被证明在AMF与宿主的共生中起重要作用。本研究以SC9木薯为研究对象，探讨茉莉酸信号通路对AMF与木薯共生体系中木薯共生建立及生长的影响。首先发现木薯与菌根真菌的共生可增加木薯地上部和地下部的生物量。其次，AMF接种初期JA含量显著增加，接种后期生长素含量显著增加，表明JA信号转导在木薯与菌根真菌的共生中起重要作用。利用转录组数据分析木薯中与JA合成和信号转导相关基因的表达差异。该基因对木薯与菌根真菌的共生呈阳性反应。对MeJAZ基因家族表达及其启动子的分析支持了这一结果。在叶片上喷施不同浓度的MeJA会影响木薯的定殖率和根生物量，表明JA是菌根形成的积极调节因子。蛋白质相互作用预测和qPCR分析表明，该基因可能是响应茉莉酸信号并调节菌根对木薯生长发育影响的关键转录调节因子。
