Luo Huan, Liu Qianqian, Chen Si, Li Xiaoli, Chen Haitao, Xia Yuanyuan, Yang Jinkui
State Key Laboratory for Conservation and Utilization of Bio-Resources, Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming 650091, China.
J Fungi (Basel). 2025 Aug 19;11(8):602. doi: 10.3390/jof11080602.
Chk1, a highly conserved serine/threonine protein kinase, functions as a critical regulator of fungal cell cycle progression, mitotic fidelity, and DNA damage response. In this study, we characterized an orthologous Chk1 (AoChk1) in a ubiquitous nematode-trapping fungus, through targeted gene knockout coupled with integrated phenotypic, metabolomic, and transcriptomic analyses. This study aims to elucidate the function and potential regulatory networks of AoChk1 in . Deletion of leads to significant reductions in nucleus number, hyphal cell length, conidial production, and trap formation, but an increase in the accumulation of lipid droplets and autophagy. In addition, transcriptomics data indicate that AoChk1 plays an important role in cell cycle and division, nuclear architecture and organelle dynamics, protein homeostasis maintenance, and membrane systems. In addition, the inactivation of the exhibited remarkably reduced metabolite abundance relative to the WT strain. In conclusion, our results identify AoChk1 as an important regulator of asexual development, pathogenicity, and metabolic processes in .
Chk1是一种高度保守的丝氨酸/苏氨酸蛋白激酶，作为真菌细胞周期进程、有丝分裂保真度和DNA损伤反应的关键调节因子发挥作用。在本研究中，我们通过靶向基因敲除结合综合表型、代谢组学和转录组学分析，对一种普遍存在的捕食线虫真菌中的直系同源Chk1（AoChk1）进行了表征。本研究旨在阐明AoChk1在……中的功能和潜在调控网络。缺失……会导致细胞核数量、菌丝细胞长度、分生孢子产生和陷阱形成显著减少，但脂滴积累和自噬增加。此外，转录组学数据表明，AoChk1在细胞周期和分裂、核结构和细胞器动态、蛋白质稳态维持以及膜系统中发挥重要作用。此外，与野生型菌株相比，……的失活表现出代谢物丰度显著降低。总之，我们的结果确定AoChk1是……中无性发育、致病性和代谢过程的重要调节因子。
