• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

线虫诱捕真菌节丛孢激活线粒体并重新编程真菌细胞以捕食线虫。

GprC of the nematode-trapping fungus Arthrobotrys flagrans activates mitochondria and reprograms fungal cells for nematode hunting.

机构信息

Department of Microbiology, Institute for Applied Biosciences, Karlsruhe Institute of Technology (KIT) - South Campus, Karlsruhe, Germany.

Department of Theoretical Chemical Biology, Institute for Physical Chemistry, Karlsruhe Institute of Technology (KIT) - South Campus, Karlsruhe, Germany.

出版信息

Nat Microbiol. 2024 Jul;9(7):1752-1763. doi: 10.1038/s41564-024-01731-9. Epub 2024 Jun 14.

DOI:10.1038/s41564-024-01731-9
PMID:38877225
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11222155/
Abstract

Initiation of development requires differential gene expression and metabolic adaptations. Here we show in the nematode-trapping fungus, Arthrobotrys flagrans, that both are achieved through a dual-function G-protein-coupled receptor (GPCR). A. flagrans develops adhesive traps and recognizes its prey, Caenorhabditis elegans, through nematode-specific pheromones (ascarosides). Gene-expression analyses revealed that ascarosides activate the fungal GPCR, GprC, at the plasma membrane and together with the G-protein alpha subunit GasA, reprograms the cell. However, GprC and GasA also reside in mitochondria and boost respiration. This dual localization of GprC in A. flagrans resembles the localization of the cannabinoid receptor CB1 in humans. The C. elegans ascaroside-sensing GPCR, SRBC66 and GPCRs of many fungi are also predicted for dual localization, suggesting broad evolutionary conservation. An SRBC64/66-GprC chimaeric protein was functional in A. flagrans, and C. elegans SRBC64/66 and DAF38 share ascaroside-binding sites with the fungal GprC receptor, suggesting 400-million-year convergent evolution.

摘要

启动发育需要差异基因表达和代谢适应。在这里，我们在食线虫真菌节丛孢菌中表明，这两者都是通过双重功能的 G 蛋白偶联受体（GPCR）实现的。节丛孢菌通过线虫特异性信息素（ascarosides）形成粘性陷阱并识别其猎物秀丽隐杆线虫。基因表达分析表明，ascarosides 在质膜上激活真菌 GPCR GprC，并与 G 蛋白α亚基 GasA 一起重新编程细胞。然而，GprC 和 GasA 也存在于线粒体中并促进呼吸。GprC 在节丛孢菌中的这种双重定位类似于大麻素受体 CB1 在人类中的定位。秀丽隐杆线虫的 ascaroside 感应 GPCR SRBC66 和许多真菌的 GPCR 也被预测为双重定位，表明广泛的进化保守性。SRBC64/66-GprC 嵌合蛋白在节丛孢菌中具有功能，并且秀丽隐杆线虫的 SRBC64/66 和 DAF38 与真菌 GprC 受体共享 ascaroside 结合位点，表明这是 4 亿年来的趋同进化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/d0fff664e467/41564_2024_1731_Fig9_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/276469e13ce4/41564_2024_1731_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/d80d99f12273/41564_2024_1731_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/b3e3e57ccd74/41564_2024_1731_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/f5ede41cf665/41564_2024_1731_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/d35acadb19ad/41564_2024_1731_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/24af66798df7/41564_2024_1731_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/e6688e2fea4a/41564_2024_1731_Fig7_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/d66e3a03cfdb/41564_2024_1731_Fig8_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/d0fff664e467/41564_2024_1731_Fig9_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/276469e13ce4/41564_2024_1731_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/d80d99f12273/41564_2024_1731_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/b3e3e57ccd74/41564_2024_1731_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/f5ede41cf665/41564_2024_1731_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/d35acadb19ad/41564_2024_1731_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/24af66798df7/41564_2024_1731_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/e6688e2fea4a/41564_2024_1731_Fig7_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/d66e3a03cfdb/41564_2024_1731_Fig8_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab97/11222155/d0fff664e467/41564_2024_1731_Fig9_ESM.jpg

相似文献

1
GprC of the nematode-trapping fungus Arthrobotrys flagrans activates mitochondria and reprograms fungal cells for nematode hunting.线虫诱捕真菌节丛孢激活线粒体并重新编程真菌细胞以捕食线虫。
Nat Microbiol. 2024 Jul;9(7):1752-1763. doi: 10.1038/s41564-024-01731-9. Epub 2024 Jun 14.
2
The nematode-trapping fungus Arthrobotrys oligospora detects prey pheromones via G protein-coupled receptors.线虫诱捕真菌节丛孢（Arthrobotrys oligospora）通过 G 蛋白偶联受体检测猎物信息素。
Nat Microbiol. 2024 Jul;9(7):1738-1751. doi: 10.1038/s41564-024-01679-w. Epub 2024 Apr 22.
3
The cysteine-rich virulence factor NipA of Arthrobotrys flagrans interferes with cuticle integrity of Caenorhabditis elegans.茂盛小克隆重泡霉富含半胱氨酸的毒力因子 NipA 会干扰秀丽隐杆线虫的表皮完整性。
Nat Commun. 2024 Jul 10;15(1):5795. doi: 10.1038/s41467-024-50096-4.
4
Natural diversity in the predatory behavior facilitates the establishment of a robust model strain for nematode-trapping fungi.捕食行为的自然多样性有助于建立一个强大的线虫诱捕真菌模式菌株。
Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6762-6770. doi: 10.1073/pnas.1919726117. Epub 2020 Mar 11.
5
AfLaeA, a Global Regulator of Mycelial Growth, Chlamydospore Production, Pathogenicity, Secondary Metabolism, and Energy Metabolism in the Nematode-Trapping Fungus .AflaeA，一种在食线虫真菌中调节菌丝生长、厚垣孢子产生、致病性、次生代谢和能量代谢的全局调控因子。
Microbiol Spectr. 2023 Aug 17;11(4):e0018623. doi: 10.1128/spectrum.00186-23. Epub 2023 Jun 26.
6
Nematophagous fungus mimics olfactory cues of sex and food to lure its nematode prey.食线虫真菌模仿性和食物的嗅觉线索来诱捕其线虫猎物。
Elife. 2017 Jan 18;6:e20023. doi: 10.7554/eLife.20023.
7
The STRIPAK component SipC is involved in morphology and cell-fate determination in the nematode-trapping fungus Duddingtonia flagrans.STRIPAK 元件 SipC 参与线虫诱捕真菌 Duddingtonia flagrans 的形态和细胞命运决定。
Genetics. 2022 Jan 4;220(1). doi: 10.1093/genetics/iyab153.
8
Paralysis of nematodes: shifts in the transcriptome of the nematode-trapping fungus Monacrosporium haptotylum during infection of Caenorhabditis elegans.线虫的麻痹：捕食线虫真菌哈氏单顶孢在感染秀丽隐杆线虫过程中的转录组变化
Environ Microbiol. 2008 Feb;10(2):364-75. doi: 10.1111/j.1462-2920.2007.01457.x. Epub 2007 Nov 19.
9
Global analysis of neuropeptide receptor conservation across phylum Nematoda.跨门纲目对神经肽受体保守性的全球分析。
BMC Biol. 2024 Oct 8;22(1):223. doi: 10.1186/s12915-024-02017-6.
10
Ascaroside expression in Caenorhabditis elegans is strongly dependent on diet and developmental stage.秀丽隐杆线虫中的阿索昔苷表达强烈依赖于饮食和发育阶段。
PLoS One. 2011 Mar 15;6(3):e17804. doi: 10.1371/journal.pone.0017804.

引用本文的文献

1
FlbD: A Regulator of Hyphal Growth, Stress Resistance, Pathogenicity, and Chlamydospore Production in the Nematode-Trapping Fungus .FlbD：一种在线虫捕捉真菌中调控菌丝生长、抗逆性、致病性和厚垣孢子产生的调节因子
Microorganisms. 2025 Aug 7;13(8):1847. doi: 10.3390/microorganisms13081847.
2
AoChk1 Is Required for Sporulation, Trap Formation, and Metabolic Process in .Aochk1是.中孢子形成、陷阱形成和代谢过程所必需的。 （原文句末的“in.”表述不完整，推测可能是某个具体物种或环境等未明确写出）
J Fungi (Basel). 2025 Aug 19;11(8):602. doi: 10.3390/jof11080602.
3
The Egh16-like virulence factor TrsA of the nematode-trapping fungus Arthrobotrys flagrans facilitates intrusion into its host Caenorhabditis elegans.

本文引用的文献

1
Key processes required for the different stages of fungal carnivory by a nematode-trapping fungus.线虫诱捕真菌捕食真菌的不同阶段所需的关键过程。
PLoS Biol. 2023 Nov 21;21(11):e3002400. doi: 10.1371/journal.pbio.3002400. eCollection 2023 Nov.
2
Ammonia and Nematode Ascaroside Are Synergistic in Trap Formation in .氨和线虫独脚金内酯在[具体情境未给出]的陷阱形成中具有协同作用。
Pathogens. 2023 Aug 31;12(9):1114. doi: 10.3390/pathogens12091114.
3
In situ architecture of the ER-mitochondria encounter structure.内质网-线粒体接触结构的原位结构。
捕食线虫真菌节丛孢菌（Arthrobotrys flagrans）中类Egh16毒力因子TrsA有助于侵入其宿主秀丽隐杆线虫。
PLoS Pathog. 2025 Aug 25;21(8):e1013370. doi: 10.1371/journal.ppat.1013370. eCollection 2025 Aug.
4
Nematode Pheromones as Key Mediators of Behavior, Development, and Ecological Interactions.线虫信息素作为行为、发育和生态相互作用的关键调节因子
Biomolecules. 2025 Jul 9;15(7):981. doi: 10.3390/biom15070981.
5
Gip1 GPCR regulates two sexual-stage differentiation processes in the ascomycete Fusarium graminearum.Gip1 G蛋白偶联受体调控子囊菌禾谷镰刀菌中的两个有性阶段分化过程。
Nat Commun. 2025 Jul 8;16(1):6279. doi: 10.1038/s41467-025-61704-2.
6
Predation by nematode-trapping fungus triggers mechanosensory-dependent quiescence in .捕食线虫真菌引发了（某种生物）中机械感觉依赖的静止状态。 （原文中“in”后面缺少具体内容）
iScience. 2025 May 30;28(7):112792. doi: 10.1016/j.isci.2025.112792. eCollection 2025 Jul 18.
7
The rise of fungal G-protein coupled receptors in pathogenesis and symbiosis.真菌G蛋白偶联受体在发病机制和共生关系中的兴起。
PLoS Pathog. 2025 Jun 12;21(6):e1013212. doi: 10.1371/journal.ppat.1013212. eCollection 2025 Jun.
8
GPCR Sense Communication Among Interaction Nematodes with Other Organisms.G蛋白偶联受体（GPCR）介导的线虫与其他生物之间的相互感应通讯。
Int J Mol Sci. 2025 Mar 20;26(6):2822. doi: 10.3390/ijms26062822.
9
Transcription Factors in Biocontrol Fungi.生防真菌中的转录因子
J Fungi (Basel). 2025 Mar 15;11(3):223. doi: 10.3390/jof11030223.
10
Fungal Stress Responses and the Importance of GPCRs.真菌应激反应与G蛋白偶联受体的重要性
J Fungi (Basel). 2025 Mar 11;11(3):213. doi: 10.3390/jof11030213.
Nature. 2023 Jun;618(7963):188-192. doi: 10.1038/s41586-023-06050-3. Epub 2023 May 10.
4
Evaluating GPCR modeling and docking strategies in the era of deep learning-based protein structure prediction.在基于深度学习的蛋白质结构预测时代评估GPCR建模和对接策略。
Comput Struct Biotechnol J. 2022 Dec 1;21:158-167. doi: 10.1016/j.csbj.2022.11.057. eCollection 2023.
5
The cAMP-PKA pathway regulates prey sensing and trap morphogenesis in the nematode-trapping fungus Arthrobotrys oligospora.cAMP-PKA 通路调控线虫诱捕真菌少孢节丛孢的猎物感知和陷阱形态发生。
G3 (Bethesda). 2022 Sep 30;12(10). doi: 10.1093/g3journal/jkac217.
6
The STRIPAK component SipC is involved in morphology and cell-fate determination in the nematode-trapping fungus Duddingtonia flagrans.STRIPAK 元件 SipC 参与线虫诱捕真菌 Duddingtonia flagrans 的形态和细胞命运决定。
Genetics. 2022 Jan 4;220(1). doi: 10.1093/genetics/iyab153.
7
AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models.AlphaFold 蛋白质结构数据库：用高精度模型极大地扩展蛋白质序列空间的结构覆盖范围。
Nucleic Acids Res. 2022 Jan 7;50(D1):D439-D444. doi: 10.1093/nar/gkab1061.
8
The small-secreted cysteine-rich protein CyrA is a virulence factor participating in the attack of Caenorhabditis elegans by Duddingtonia flagrans.小分子富含半胱氨酸的蛋白 CyrA 是一种毒力因子，参与了多形汉逊酵母对秀丽隐杆线虫的攻击。
PLoS Pathog. 2021 Nov 4;17(11):e1010028. doi: 10.1371/journal.ppat.1010028. eCollection 2021 Nov.
9
Fatal attraction of Caenorhabditis elegans to predatory fungi through 6-methyl-salicylic acid.通过 6-甲基水杨酸，秀丽隐杆线虫对捕食性真菌的致命吸引力。
Nat Commun. 2021 Sep 15;12(1):5462. doi: 10.1038/s41467-021-25535-1.
10
AutoDock Vina 1.2.0: New Docking Methods, Expanded Force Field, and Python Bindings.AutoDock Vina 1.2.0：新的对接方法、扩展的力场及Python绑定
J Chem Inf Model. 2021 Aug 23;61(8):3891-3898. doi: 10.1021/acs.jcim.1c00203. Epub 2021 Jul 19.