Niu Tingting, Qian Haike, Cheng Lufan, Luo Qijun, Chen Juanjuan, Yang Rui, Zhang Peng, Wang Tiegan, Chen Haimin
Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China.
Zhejiang Mariculture Research Institute, Wenzhou 325005, China.
Mar Drugs. 2025 Jul 30;23(8):309. doi: 10.3390/md23080309.
The transition from conchocelis to conchosporangia in represents a pivotal stage in its life cycle. As a commercially vital red alga, plays a dominant role in global nori production. The transition governing its sporulation efficiency is pivotal for aquaculture success, yet the underlying regulatory mechanisms, especially their integration with metabolic cues such as polyamines, remain poorly understood. This study uncovered a critical role for the polyamine spermine (SPM) in promoting conchosporangial formation, mediated through the signaling activity of superoxide anions (O·). Treatment with SPM markedly elevated O· levels, an effect that was effectively inhibited by the NADPH oxidase inhibitor diphenyliodonium chloride (DPI), underscoring the role of O· as a key signaling molecule. Transcriptomic analysis revealed that SPM enhanced photosynthesis, carbon assimilation, and respiratory metabolism, while simultaneously activating antioxidant enzymes, such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT), to regulate hydrogen peroxide (HO) levels and maintain redox homeostasis. Furthermore, SPM upregulated genes associated with photosynthetic carbon fixation and the C oxidative photorespiration pathway, supplying the energy and metabolic resources necessary for this developmental transition. These findings suggested that SPM orchestrated O· signaling, photosynthetic activity, and antioxidant defenses to facilitate the transition from conchocelis to conchosporangia in .
在[具体物种]中，从壳孢子体到壳孢子囊的转变是其生命周期中的一个关键阶段。作为一种具有重要商业价值的红藻，[具体物种]在全球紫菜生产中占据主导地位。控制其孢子形成效率的转变对于水产养殖的成功至关重要，然而其潜在的调控机制，尤其是它们与多胺等代谢信号的整合，仍知之甚少。本研究发现多胺精胺（SPM）在促进壳孢子囊形成中起关键作用，这一作用是通过超氧阴离子（O·）的信号活性介导的。用SPM处理显著提高了O·水平，NADPH氧化酶抑制剂氯化二苯基碘鎓（DPI）有效地抑制了这一效应，突出了O·作为关键信号分子的作用。转录组分析表明，SPM增强了光合作用、碳同化和呼吸代谢，同时激活了抗氧化酶，如超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）和过氧化氢酶（CAT），以调节过氧化氢（H₂O₂）水平并维持氧化还原稳态。此外，SPM上调了与光合碳固定和C₂氧化光呼吸途径相关的基因，为这一发育转变提供了必要的能量和代谢资源。这些发现表明，SPM协调了O·信号传导、光合活性和抗氧化防御，以促进[具体物种]从壳孢子体到壳孢子囊的转变。
