Krawczyk Aleksandra, Rycerz Karol, Jaworska-Adamu Jadwiga, Arciszewski Marcin B
Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland.
Biomolecules. 2025 Aug 1;15(8):1111. doi: 10.3390/biom15081111.
The habenula (Hb) of the epithalamus is formed of the medial (MHb) and lateral (LHb) parts. The improper functioning of the Hb may lead to depression and anxiety. The glutamate excitotoxicity is accompanied by astroglia reactivity and leads to the damage of nervous system structures. The aim of the study was to assess the influence of monosodium glutamate (MSG) administrated subcutaneously to rats in doses of 2 g/kg b.w. (I) and 4 g/kg b.w. (II), on astroglia in the MHb and LHb. Based on immunohistochemical reactions, the morphology, number of astrocytes immunoreactive for glial fibrillary acidic protein (GFAP-IR) and S100β protein (S100β-IR), and their surface area, perimeter, number and length of processes, and cytoplasmic-nuclear immunostaining intensity for the studied proteins were assessed. In the MHb of animals receiving MSG, especially at a high dose, hypertrophy and an increase in the number of GFAP-IR and S100β-IR cells were demonstrated. In the LHb, only hypertrophy of processes in S100β-positive glia was observed. The immunostaining intensity increased in GFAP-IR glia and decreased in S100β-IR cells only in animals from group I. The results revealed that astroglia respond to MSG depending on its dose and the Hb part. This different behavior of glia may indicate their different sensitivity and resistance to damaging factors.
上丘脑的缰核（Hb）由内侧缰核（MHb）和外侧缰核（LHb）组成。缰核功能异常可能导致抑郁和焦虑。谷氨酸兴奋性毒性伴随着星形胶质细胞反应，并导致神经系统结构损伤。本研究的目的是评估以2 g/kg体重（I组）和4 g/kg体重（II组）的剂量皮下注射给大鼠的味精（MSG）对内侧缰核和外侧缰核中星形胶质细胞的影响。基于免疫组织化学反应，评估了对胶质纤维酸性蛋白（GFAP-IR）和S100β蛋白（S100β-IR）免疫反应阳性的星形胶质细胞的形态、数量，以及它们的表面积、周长、突起数量和长度，还有所研究蛋白质的细胞质-细胞核免疫染色强度。在接受味精的动物的内侧缰核中，尤其是高剂量时，出现了GFAP-IR和S100β-IR细胞肥大和数量增加的情况。在外侧缰核中，仅观察到S100β阳性胶质细胞的突起肥大。仅在I组动物中，GFAP-IR胶质细胞的免疫染色强度增加，而S100β-IR细胞的免疫染色强度降低。结果表明，星形胶质细胞对味精的反应取决于其剂量和缰核部分。胶质细胞的这种不同行为可能表明它们对损伤因素的敏感性和抵抗力不同。
