Magaña-Rodríguez Oliver Rafid, Ortega-Pérez Luis Gerardo, García-Calderón Aram Josué, Ayala-Ruiz Luis Alberto, Piñón-Simental Jonathan Saúl, Aguilera-Méndez Asdrubal, Godínez-Hernández Daniel, Rios-Chavez Patricia
Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Mexico.
Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Mexico.
Biomolecules. 2025 Aug 5;15(8):1129. doi: 10.3390/biom15081129.
has shown antioxidant and anti-inflammatory properties in certain tissues. However, its impact on the brain remains unproven. This study investigates the effect of extract and phytosomes on the oxidative status of the brains of rats fed a high-fat-fructose diet (HFD). Fifty-four male Wistar rats were randomly divided into nine groups ( = 6). Groups 1, 2, and 3 received a standard chow diet; Group 2 also received the vehicle, and Group 3 was supplemented with extract (200 mg/kg). Groups 4, 5, 6, 7, 8, and 9 received a high-fat diet (HFD). Additionally, groups 5, 6, 7, 8, and 9 were supplemented with orlistat at 5 mg/kg, extract at 200 mg/kg, and phytosomes loaded with at doses of 50, 100, and 200 mg/kg, respectively. Administration was oral for 16 weeks. Antioxidant enzymes, biomarkers of oxidative stress, and fatty acid content in the brain were determined. A parallel artificial membrane permeability assay (PAMPA) was employed to identify compounds that can cross the intestinal and blood-brain barriers. The HFD group (group 4) increased body weight and adipose tissue, unlike the other groups. The brain fatty acid profile showed slight variations in all of the groups. On the other hand, group 4 showed a decrease in the activities of antioxidant enzymes SOD, CAT, and PON. It reduced GSH level, while increasing GPx activity as well as MDA, 4-HNE, and AOPP levels. extract and phytosomes restore the antioxidant enzyme activities and mitigate oxidative stress in the brain. modulates oxidative stress in brain tissue through 1.8-cineole and α-terpineol, which possess antioxidant and anti-inflammatory properties.
已在某些组织中显示出抗氧化和抗炎特性。然而，其对大脑的影响仍未得到证实。本研究调查了提取物和磷脂体对喂食高脂果糖饮食（HFD）的大鼠大脑氧化状态的影响。54只雄性Wistar大鼠随机分为9组（每组n = 6）。第1、2和3组给予标准饲料；第2组还接受赋形剂，第3组补充提取物（200 mg/kg）。第4、5、6、7、8和9组给予高脂饮食（HFD）。此外，第5、6、7、8和9组分别补充5 mg/kg的奥利司他、200 mg/kg的提取物以及剂量为50、100和200 mg/kg的负载提取物的磷脂体。口服给药16周。测定大脑中的抗氧化酶、氧化应激生物标志物和脂肪酸含量。采用平行人工膜通透性测定法（PAMPA）来鉴定可穿过肠道和血脑屏障的化合物。与其他组不同，高脂饮食组（第4组）体重和脂肪组织增加。所有组的大脑脂肪酸谱均有轻微变化。另一方面，第4组抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和对氧磷酶（PON）的活性降低。它降低了谷胱甘肽（GSH）水平，同时增加了谷胱甘肽过氧化物酶（GPx）活性以及丙二醛（MDA）、4-羟基壬烯醛（4-HNE）和晚期氧化蛋白产物（AOPP）水平。提取物和磷脂体可恢复大脑中的抗氧化酶活性并减轻氧化应激。提取物通过具有抗氧化和抗炎特性的1,8-桉叶素和α-松油醇调节脑组织中的氧化应激。
