Imm Seulgi, Bai Jaewoo, Chang Yoonjee
Department of Food and Nutrition, College of Science and Technology, Kookmin University, Seoul 02707, Republic of Korea.
Food Science and Technology, Seoul Women's University, Seoul 01797, Republic of Korea.
Int J Mol Sci. 2025 Aug 12;26(16):7793. doi: 10.3390/ijms26167793.
Biodegradable poly(lactic acid) (PLA)/poly(ε-caprolactone) (PCL) composite films were prepared with a compatibilizer (tributyl citrate, TBC) using a solvent casting method. Incorporation of 5% TBC (/, of PCL weight) improved tensile strength and elongation at break (21.93 ± 2.33 MPa and 21.02 ± 1.54%, respectively) and reduced water vapor permeability (from 0.12 ± 0.01 to 0.098 ± 0.01 g·mm·m·h·kPa), indicating improved compatibility between PLA and PCL. phage PBSA08 demonstrated rapid and persistent bacteriolytic activity for up to 24 h, suggesting its potential as a promising antibacterial biological agent. To impart antibacterial properties to the developed PLA/PCL/TBC film, PBSA08 was loaded into sodium alginate (SA) and coated on the film surface. The optimal composition was 3% (/) SA and 3% (/) glycerol, which exhibited suitable dynamic behavior as a coating solution and excellent adhesion to the film surface. The phage-coated antibacterial films demonstrated progressive and significant inhibition against starting from 10 to 24 h, with controlled phage-release properties. Overall, the developed active film might exert sustained and remarkable antibacterial effects through controlled release of biological agents (phage) under realistic packaging conditions.
采用溶液浇铸法，使用增容剂（柠檬酸三丁酯，TBC）制备了可生物降解的聚乳酸（PLA）/聚己内酯（PCL）复合薄膜。加入5%的TBC（基于PCL重量）提高了拉伸强度和断裂伸长率（分别为21.93±2.33兆帕和21.02±1.54%），并降低了水蒸气透过率（从0.12±0.01降至0.098±0.01克·毫米·平方米·小时·千帕），表明PLA和PCL之间的相容性得到改善。噬菌体PBSA08在长达24小时内表现出快速且持久的溶菌活性，表明其作为一种有前景的抗菌生物制剂的潜力。为了赋予所制备的PLA/PCL/TBC薄膜抗菌性能，将PBSA08负载到海藻酸钠（SA）中并涂覆在薄膜表面。最佳组成是3%（基于重量）SA和3%（基于重量）甘油，其作为涂层溶液表现出合适的动态行为以及对薄膜表面的优异附着力。噬菌体包被的抗菌薄膜从10到24小时对[具体细菌]表现出渐进且显著的抑制作用，并具有可控的噬菌体释放特性。总体而言，所制备的活性薄膜在实际包装条件下可能通过生物制剂（噬菌体）的控释发挥持续且显著的抗菌效果。
