Poumay Y, Ronveaux-Dupal M F
J Lipid Res. 1985 Dec;26(12):1476-80.
In order to study cellular metabolism of low density lipoproteins (LDL), ultracentrifugal methods have been used to isolate the lipoproteins. The use of vertical rotor ultracentrifugation very quickly produces small quantities of diluted lipoproteins per gradient, as well as small volumes of lipoprotein-deficient serum. We present modifications to this method in order to prepare routinely more concentrated LDL and a sufficient volume of lipoprotein-deficient serum in a relatively short time with minimal cost and handling.
为了研究低密度脂蛋白（LDL）的细胞代谢，已采用超速离心法分离脂蛋白。使用垂直转子超速离心法，每个梯度能很快产生少量稀释的脂蛋白，以及少量脂蛋白缺乏血清。我们对该方法进行了改进，以便在相对较短的时间内，以最低的成本和最少的操作，常规制备出更浓缩的LDL和足够量的脂蛋白缺乏血清。
