呼吸道合胞病毒细支气管炎患儿血液和颊黏膜中NF-κB mRNA的差异表达

Differential NF-κB mRNA Expression in Blood and Buccal Mucosa of Pediatric Patients with RSV Bronchiolitis.

作者信息

Savino Francesco, Calvi Cristina, Gambarino Stefano, Dini Maddalena, Pau Anna, Montanari Paola, Clemente Anna, Galliano Ilaria, Bergallo Massimiliano

机构信息

Early Infancy Special Care Unit, Regina Margherita Children Hospital, A.O.U. Città della Salute e della Scienza di Torino, 10126 Turin, Italy.

Pediatric Laboratory, Department of Public Health and Pediatric Sciences, University of Turin, Regina Margherita Children's Hospital, 10126 Turin, Italy.

出版信息

Genes (Basel). 2025 Jul 22;16(8):851. doi: 10.3390/genes16080851.

DOI:10.3390/genes16080851

PMID:40869899

Abstract

BACKGROUND

Respiratory syncytial virus (RSV) bronchiolitis is a leading cause of lower respiratory tract infections in children under two years of age. NF-κB is a key transcription factor in antiviral and inflammatory responses. This study investigates the expression of NF-κB mRNA in both blood and buccal swab samples of pediatric patients hospitalized for RSV bronchiolitis, comparing levels at admission and discharge.

METHODS

Paired peripheral blood and buccal swab samples were collected from pediatric patients ( = 85) at hospital admission and discharge. Quantitative real-time PCR was used to assess NF-κB mRNA levels.

RESULTS

NF-κB mRNA levels significantly decreased in blood between admission and discharge ( < 0.05), while no significant change was observed in buccal swabs.

CONCLUSIONS

These results suggest a compartment-specific regulation of NF-κB, with systemic inflammatory resolution at discharge and persistent or distinct mucosal immune activity. Understanding these dynamics may improve our approach to monitoring and treating RSV bronchiolitis.

摘要