核糖体休眠因子将群体感应与肠出血性大肠杆菌的耐酸性联系起来。
Ribosomal Hibernation Factor Links Quorum-Sensing to Acid Resistance in EHEC.
作者信息
Yang Yang, Zhang Xinyi, Han Zixin, Li Junpeng, Fang Qiaoqiao, Zhu Guoqiang
机构信息
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Joint Laboratory of International Cooperation on Prevention and Control Technology of Important Animal Diseases and Zoonoses of Jiangsu, Yangzhou 225009, China.
出版信息
Microorganisms. 2025 Jul 24;13(8):1730. doi: 10.3390/microorganisms13081730.
The mechanism by which quorum sensing (QS) enhances stress resistance in enterohemorrhagic () O157:H7 remains unclear. We employed optimized exogenous QS signal N-acyl-homoserinelactones (AHL) (100 μM 3-oxo-C6-AHL, 2 h) in EHEC O157:H7 strain EDL933, which was validated with endogenous -derived AHL, to investigate QS-mediated protection against acid stress. RNA-seq transcriptomics identified key upregulated genes (e.g., ). Functional validation using isogenic knockout mutants generated via λ-Red demonstrated abolished stress resistance and pan-stress vulnerability. Mechanistic studies employing qRT-PCR and stress survival assays established Ribosomal Hibernation Factor (RMF) as a non-redundant executor in a SdiA-RMF-RpoS axis, which activates ribosomal dormancy and SOS response to enhance EHEC survival under diverse stresses. For the first time, we define ribosomal hibernation as the core adaptive strategy linking QS to pathogen resilience, providing crucial mechanistic insights for developing EHEC control measures against foodborne threats.
群体感应（QS）增强肠出血性大肠杆菌（EHEC）O157:H7抗逆性的机制尚不清楚。我们在EHEC O157:H7菌株EDL933中使用了优化的外源性QS信号N-酰基高丝氨酸内酯（AHL）（100μM 3-氧代-C6-AHL，2小时），并用内源性AHL进行了验证，以研究QS介导的抗酸应激保护作用。RNA测序转录组学确定了关键上调基因（如……）。使用通过λ-Red产生的同基因缺失突变体进行的功能验证表明，抗逆性丧失且对多种应激敏感。采用定量逆转录聚合酶链反应（qRT-PCR）和应激存活试验的机制研究确定核糖体休眠因子（RMF）是SdiA-RMF-RpoS轴中的一个非冗余执行者，该轴激活核糖体休眠和SOS反应，以增强EHEC在多种应激下的存活能力。我们首次将核糖体休眠定义为将QS与病原体恢复力联系起来的核心适应性策略，为制定针对食源性病原体威胁的EHEC控制措施提供了关键的机制见解。
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