来自圈养大熊猫和小熊猫携带与移动遗传元件相关的多种抗菌抗性基因和毒力基因。

from Captive Giant Pandas and Red Pandas Carries Diverse Antimicrobial Resistance Genes and Virulence Genes Associated with Mobile Genetic Elements.

作者信息

Yang Yizhou, Liu Yan, Wang Jiali, Li Caiwu, Wu Ruihu, Xin Jialiang, Yang Xue, Zheng Haohong, Zhong Zhijun, Fu Hualin, Zhou Ziyao, Liu Haifeng, Peng Guangneng

机构信息

Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Beijing Key Laboratory of Captive Wildlife Technologies, Beijing Zoo, Beijing 100044, China.

出版信息

Microorganisms. 2025 Aug 1;13(8):1802. doi: 10.3390/microorganisms13081802.

DOI:10.3390/microorganisms13081802

PMID:40871306

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12388390/

Abstract

is a zoonotic pathogen that poses a growing threat to both animal and human health due to rising antimicrobial resistance (AMR). It is widely found in animals, including China's nationally protected captive giant and red pandas. This study isolated from panda feces to assess AMR and virulence traits, and used whole-genome sequencing (WGS) to evaluate the spread of resistance genes (ARGs) and virulence genes (VAGs). In this study, 37 isolates were obtained, 20 from red pandas and 17 from giant pandas. Multidrug-resistant (MDR) strains were present in both hosts. Giant panda isolates showed the highest resistance to ampicillin and cefazolin (58.8%), while red panda isolates were most resistant to trimethoprim/sulfamethoxazole (65%) and imipenem (55%). Giant panda-derived strains also exhibited stronger biofilm formation and swarming motility. WGS identified 31 ARGs and 73 VAGs, many linked to mobile genetic elements (MGEs) such as plasmids, integrons, and ICEs. In addition, we found frequent co-localization of drug resistance genes/VAGs with MGEs, indicating a high possibility of horizontal gene transfer (HGT). This study provides crucial insights into AMR and virulence risks in from captive pandas, supporting targeted surveillance and control strategies.

摘要

是一种人畜共患病原体，由于抗菌药物耐药性（AMR）不断上升，对动物和人类健康构成日益严重的威胁。它在动物中广泛存在，包括中国国家保护的圈养大熊猫和小熊猫。本研究从熊猫粪便中分离出[病原体名称未给出]以评估AMR和毒力特征，并使用全基因组测序（WGS）来评估耐药基因（ARGs）和毒力基因（VAGs）的传播情况。在本研究中，获得了37株分离株，其中20株来自小熊猫，17株来自大熊猫。两个宿主中均存在多重耐药（MDR）菌株。大熊猫分离株对氨苄西林和头孢唑林的耐药性最高（58.8%），而小熊猫分离株对甲氧苄啶/磺胺甲恶唑（65%）和亚胺培南（55%）的耐药性最强。大熊猫来源的菌株还表现出更强的生物膜形成能力和群集运动性。WGS鉴定出31个ARGs和73个VAGs，许多与质粒、整合子和ICEs等移动遗传元件（MGEs）相关。此外，我们发现耐药基因/VAGs与MGEs频繁共定位，表明水平基因转移（HGT）的可能性很高。本研究为圈养大熊猫的AMR和毒力风险提供了关键见解，支持有针对性的监测和控制策略。