Fukuoka Industrial Technology Center, Biotechnology and Food Research Institute, 1465-5 Aikawamachi, Kurume, 839-0861, Japan.
World J Microbiol Biotechnol. 2022 Jan 6;38(2):24. doi: 10.1007/s11274-021-03206-z.
The ethanol extracts of 155 different foodstuffs containing medicinal plants were investigated for their biofilm eradication activities against pathogenic bacteria. A combined method of a colorimetric microbial viability assay based on reduction of a tetrazolium salt (WST-8) and a biofilm formation technique on the 96-pins of a microtiter plate lid was used to screen the biofilm eradication activities of foodstuffs. The ethanol extracts of licorice (Glycyrrhiza glabra) showed potent biofilm eradication activities against Streptococcus mutans, Staphylococcus aureus, and Porphyromonas gingivalis. Among the antimicrobial constituents in licorice, glabridin had the most potent eradication activities against microbial biofilms. The minimum biofilm eradication concentration of glabridin was 25-50 μg/ml. Furthermore, the combination of glabridin with ɛ-poly-L-lysine, a food additive, could result in broad biofilm eradication activities towards a wide variety of bacteria associated with infection, including Escherichia coli and Pseudomonas aeruginosa.
对含有药用植物的 155 种不同食品的乙醇提取物进行了生物膜消除活性研究，以对抗致病菌。采用基于四唑盐（WST-8）还原的比色微生物活力测定法和 96 孔微量滴定板盖的生物膜形成技术相结合的方法筛选食品的生物膜消除活性。甘草（Glycyrrhiza glabra）的乙醇提取物对变形链球菌、金黄色葡萄球菌和牙龈卟啉单胞菌表现出很强的生物膜消除活性。在甘草中的抗菌成分中，甘草素对微生物生物膜具有最强的消除活性。甘草素的最低生物膜消除浓度为 25-50μg/ml。此外，将甘草素与食品添加剂ε-聚赖氨酸结合使用，可对与感染相关的多种细菌（包括大肠杆菌和铜绿假单胞菌）产生广泛的生物膜消除活性。
