膳食铁摄入量影响小鼠肠道和肺部微生物群的微生物组成。

Dietary Iron Intake Impacts the Microbial Composition of the Murine Intestinal and Lung Microbiome.

作者信息

Essilfie Ama-Tawiah, Smith Alison, Watts Rebecca, Maniam Pramila, Lamont Iain L, Frazer David M, Anderson Gregory J, Reid David W

机构信息

QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia.

The School of Medicine, Herston Campus, The University of Queensland, Brisbane, QLD 4006, Australia.

出版信息

Nutrients. 2025 Aug 20;17(16):2696. doi: 10.3390/nu17162696.

DOI:10.3390/nu17162696

PMID:40871723

Abstract

BACKGROUND

Iron is an essential nutrient for many bacterial pathogens and normal cellular function and homeostasis of their hosts. Studies suggest that iron deficiency or overload may contribute to the pathogenesis of several chronic conditions and modify host-microbial interactions. In this study, we assessed the impact of varying dietary iron intakes on the microbiota of the intestinal tract and lungs of wild-type mice.

METHODS

Male C57BL/6J mice were fed either a standard pellet chow (high iron diet), a ferrous ammonium sulfate (FeAS)-supplemented diet or an iron-deficient diet for four weeks. Tissue from the lung, duodenum and colon was collected, and 16S rRNA gene fragments were pyrosequenced.

RESULTS

Total serum iron levels were negatively associated with richness of the lung microbiome ( = 0.035). In the murine lungs, there was no association between the iron diet and the overall lung microbiota community composition, but spp. were significantly enriched in the lungs of mice fed the FeAS diet (LDA score > 4, < 0.05). The community composition of the intestinal microbiota changed significantly depending on the iron diet, with increased richness in the low-iron compared to the iron-supplemented groups ( = 0.053). In the duodenum, spp. were reduced (Mean = 7.869, SEM = 3.464, < 0.05), and species increased (Mean = 5.343, SEM = 1.362, < 0.001) in iron-supplemented groups compared to the low-iron-diet group. In the colon, and species were reduced (Mean = 7.175, SEM = 2.246, < 0.01 and Mean = 6.967, SEM = 1.834, < 0.01 respectively), and increased (Mean = 24.03, SEM = 8.919, < 0.05) in mice on higher-iron diets compared to the low-iron diet.

DISCUSSION

This study demonstrates that dietary iron intake significantly impacts the intestinal microbiota and has a small, yet significant, effect on the lung microbiome in C57BL/6J mice. Whilst dietary iron content per se did not significantly modulate the composition of the lung microbiota, serum iron levels had subtle impacts on the community composition of the lung microbiota.

摘要

背景

铁是许多细菌病原体以及其宿主正常细胞功能和体内平衡所必需的营养素。研究表明，缺铁或铁过载可能导致几种慢性疾病的发病机制，并改变宿主与微生物的相互作用。在本研究中，我们评估了不同饮食铁摄入量对野生型小鼠肠道和肺部微生物群的影响。

方法

雄性C57BL/6J小鼠分别喂食标准颗粒饲料（高铁饮食）、补充硫酸亚铁铵（FeAS）的饮食或缺铁饮食四周。收集肺、十二指肠和结肠组织，并对16S rRNA基因片段进行焦磷酸测序。

结果

血清总铁水平与肺部微生物群的丰富度呈负相关（ = 0.035）。在小鼠肺部，铁饮食与整体肺部微生物群群落组成之间没有关联，但在喂食FeAS饮食的小鼠肺部，属显著富集（线性判别分析得分>4， < 0.05）。肠道微生物群的群落组成因铁饮食而异，与铁补充组相比，低铁组的丰富度增加（ = 0.053）。在十二指肠中，与低铁饮食组相比，铁补充组中属减少（平均值 = 7.869，标准误 = 3.464， < 0.05），种增加（平均值 = 5.343，标准误 = 1.362， < 0.001）。在结肠中，与低铁饮食相比，高铁饮食的小鼠中种和种减少（平均值分别为 = 7.175，标准误 = 2.246， < 0.01和平均值 = 6.967，标准误 = 1.834， < 0.01），增加（平均值 = 24.03，标准误 = 8.919， < 0.05）。