马铃薯中基因家族的鉴定与特征分析

Identification and Characterization of the Gene Family in Potato.

作者信息

Wang Zhiqi, Wu Wenbo, Liu Tao, Shi Wenting, Ma Kai, He Zhouwen, Chen Lixuan, Du Chong, Wang Chaonan, Yang Zhongmin

机构信息

College of Horticulture, Xinjiang Agricultural University, Urumqi 830052, China.

Postdoctoral Station of Horticulture, Xinjiang Agricultural University, Urumqi 830052, China.

出版信息

Plants (Basel). 2025 Aug 9;14(16):2472. doi: 10.3390/plants14162472.

DOI:10.3390/plants14162472

PMID:40872095

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12388987/

Abstract

Carboxypeptidase A inhibitor (CPAI) is a globular polypeptide that specifically inhibits carboxypeptidase A activity in the insect gut, playing a vital role in plant defense against external stimuli. To date, this gene family has not been systematically characterized in potatoes. In this study, we identified the gene family using the potato DM v6.1 genome and analyzed genomic and amino acid sequence features. Results demonstrated that eight members in potatoes share high homology with orthologs in tomatoes, eggplants, and peppers. Their promoter regions contain predicted cis-acting elements associated with defense and stress responses. Additionally, qRT-PCR analysis revealed elevated expression of specific members in tubers and aerial tubers, with concurrent responses to auxin treatment. These findings provide a foundation for elucidating the roles of genes in potato development.

摘要

羧肽酶A抑制剂（CPAI）是一种球状多肽，可特异性抑制昆虫肠道中的羧肽酶A活性，在植物抵御外部刺激中发挥重要作用。迄今为止，该基因家族尚未在马铃薯中得到系统表征。在本研究中，我们利用马铃薯DM v6.1基因组鉴定了该基因家族，并分析了基因组和氨基酸序列特征。结果表明，马铃薯中的8个成员与番茄、茄子和辣椒中的直系同源基因具有高度同源性。它们的启动子区域包含与防御和应激反应相关的预测顺式作用元件。此外，qRT-PCR分析显示，特定成员在块茎和气生块茎中的表达升高，同时对生长素处理有反应。这些发现为阐明该基因在马铃薯发育中的作用提供了基础。