Fabio-Braga Ana, Salois Jaida, Bryant Mitchell L, Kollath Daniel R, Barker Bridget
Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA.
Pathogens. 2025 Aug 14;14(8):807. doi: 10.3390/pathogens14080807.
Microbiome studies report a decrease in diversity associated with active infections. Under the endozoan hypothesis, can inhabit a host without causing disease. In this study, we describe and compare the lung mycobiome of -positive and -negative samples obtained from wildlife. If is not causing infection, we predict there will be no differences in the mycobiome between positive and negative samples. Lung samples were obtained from mammals previously trapped in Tucson, Arizona, USA ( = 26), and Mesa, Arizona, USA ( = 14). Samples were screened for with CocciDx, and mycobiome was characterized through Illumina-based amplicon sequencing of the internal transcribed spacer 2 (ITS2). We compared alpha and beta diversity of the mycobiome to assess the effects of presence and host taxonomy. A greater number of reads were captured from Tucson samples (114,706.4 ± 57,945.8) than from Mesa (384.9 ± 953.5); however, Mesa (16.8 ± 8.8) and Tucson (12 ± 7.8) had a similar number of fungal genera per sample. CocciDx detected in more samples than the ITS2 amplicon sequencing. All samples from Mesa and five from Tucson tested positive for . Therefore, Mesa samples were excluded from statistical analysis. No difference in alpha and beta diversity was associated with presence, which is consistent with the endozoan hypothesis. Host taxonomy had a significant effect on beta diversity. This effect is likely driven by host behavioral and physiological differences.
微生物组研究报告称，与活动性感染相关的多样性会降低。在内生动物假说下，[此处原文缺失相关生物名称]可以寄生于宿主而不引发疾病。在本研究中，我们描述并比较了从野生动物获取的[此处原文缺失相关生物名称]阳性和阴性样本的肺部真菌微生物组。如果[此处原文缺失相关生物名称]未引发感染，我们预测阳性和阴性样本之间的真菌微生物组不会有差异。肺部样本取自先前在美国亚利桑那州图森市捕获的哺乳动物（n = 26）以及美国亚利桑那州梅萨市捕获的哺乳动物（n = 14）。样本用CocciDx进行[此处原文缺失相关检测内容]筛查，通过基于Illumina的内转录间隔区2（ITS2）扩增子测序对真菌微生物组进行表征。我们比较了真菌微生物组的α多样性和β多样性，以评估[此处原文缺失相关生物名称]存在及宿主分类学的影响。从图森市样本中捕获的读数（114,706.4 ± 57,945.8）比梅萨市样本（384.9 ± 953.5）更多；然而，梅萨市（16.8 ± 8.8）和图森市（12 ± 7.8）每个样本的真菌属数量相似。CocciDx检测到[此处原文缺失相关生物名称]的样本比ITS2扩增子测序更多。来自梅萨市的所有样本以及来自图森市的五个样本检测出[此处原文缺失相关生物名称]呈阳性。因此，梅萨市样本被排除在统计分析之外。α多样性和β多样性与[此处原文缺失相关生物名称]的存在无关，这与内生动物假说一致。宿主分类学对β多样性有显著影响。这种影响可能是由宿主行为和生理差异驱动的。
