Martin X, Opgennorth T J, Werness P G, Rundquist R T, Romero J C, Smith L H
Kidney Int. 1985 Oct;28(4):636-9. doi: 10.1038/ki.1985.176.
Differences in calcium oxalate crystal growth inhibition were studied in normally voided urine (bladder urine) and in urine collected directly from the kidney (kidney urine) in nine dogs. Urine samples were collected before and 10 days after bilateral ureterostomies. Calcium oxalate crystal growth inhibition was measured in a standard seeded crystal growth system. The alcian blue-precipitable material of the urine samples was determined. Significantly lower values were observed in kidney urine than in bladder urine for calcium oxalate crystal inhibition (mean difference, 0.07 +/- 0.02 inhibitor units/mg creatinine; P less than 0.01) and for the alcian blue-precipitable material (mean difference, 0.07 +/- 0.02 mg/mg creatinine; P less than 0.01). We conclude that the bladder adds calcium oxalate crystal growth inhibition to urine. Glycosaminoglycans from the bladder mucosa may be responsible; however, other acidic polymers such as RNA fragments or glycopeptides have been shown to be a constituent of the alcian blue-precipitable material. These are potent inhibitors of calcium oxalate crystal growth, and their participation in the increase of inhibition observed in bladder urine cannot be excluded. Total calcium oxalate crystal growth inhibition present in normally voided urine may be an overestimation of the actual inhibition present at the level of the kidney, where calculi usually form.
在9只犬中，研究了正常排尿的尿液（膀胱尿液）和直接从肾脏收集的尿液（肾脏尿液）中草酸钙晶体生长抑制的差异。在双侧输尿管造口术前和术后10天收集尿液样本。在标准接种晶体生长系统中测量草酸钙晶体生长抑制。测定尿液样本中阿尔辛蓝可沉淀物质。草酸钙晶体抑制方面，肾脏尿液中的值显著低于膀胱尿液（平均差异为0.07±0.02抑制剂单位/毫克肌酐；P<0.01），阿尔辛蓝可沉淀物质方面也是如此（平均差异为0.07±0.02毫克/毫克肌酐；P<0.01）。我们得出结论，膀胱向尿液中添加了草酸钙晶体生长抑制物质。膀胱黏膜中的糖胺聚糖可能起作用；然而，其他酸性聚合物，如RNA片段或糖肽，已被证明是阿尔辛蓝可沉淀物质的组成部分。这些是草酸钙晶体生长的有效抑制剂，不能排除它们参与膀胱尿液中观察到的抑制增加。正常排尿尿液中存在的总草酸钙晶体生长抑制可能高估了结石通常形成部位——肾脏水平实际存在的抑制。
