利用光栅耦合干涉测量法研究肌醇焦磷酸-蛋白质相互作用。
The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate-Protein Interactions.
作者信息
Sturm Kristina, Hothorn Michael
机构信息
Structural Plant Biology Laboratory, Department of Plant Sciences, University of Geneva, Geneva, Switzerland.
出版信息
Methods Mol Biol. 2025;2972:153-169. doi: 10.1007/978-1-0716-4799-8_12.
PMID:40879985
Abstract
Grating-coupled interferometry (GCI) is a biophysical method suitable to studying the binding kinetics of protein-ligand interactions. Here, we describe the application of biotinylated inositol pyrophosphate (PP-InsP) nutrient messengers to characterize PP-InsP-protein interactions using a Creoptix WAVEsystem.
摘要
光栅耦合干涉测量法（GCI）是一种适用于研究蛋白质-配体相互作用结合动力学的生物物理方法。在此，我们描述了使用Creoptix WAVE系统，应用生物素化的肌醇焦磷酸（PP-InsP）营养信使来表征PP-InsP-蛋白质相互作用。
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