A chromosomal-level genome assembly of Omiodes indicata Fabricius (Lepidoptera: Crambidae).

Omiodes indicata, a significant pest of legumes, impacts food security in tropical and subtropical regions of Asia, Africa, and the Americas Asia. However, the lack of high-quality genomes has limited our understanding of the ecology of O. indicata. In this study, we present a high-quality genome assembly of O. indicata generated using advanced sequencing technologies, including PacBio HiFi long reads, Illumina short-read, and Hi-C platforms. The final assembly spans 493.08 Mb, comprising 59 scaffolds (scaffold N50: 17.25 Mb) and 100 contigs (contig N50: 15.72 Mb), with 99.80% of the total assembly (492.12 Mb) successfully anchored to 31 chromosomes. BUSCO analysis (n = 1,367) indicates a high level of completeness, with 99.1% of genes detected: 96.6% as single-copy and 2.5% as duplicated. Repetitive elements constitute 38.13% (188.00 Mb) of the genome, and 14,713 protein-coding genes were predicted. The high-quality O. indicata genome represents a valuable resource for diverse molecular ecology studies and will contribute to the advancement of modern pest management strategies.