Yan Hui-Jiao, Li Ming-Ye, Zhou Jia-Le, Geng Yan-Ling, Wang Xiao
Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
J Nat Prod. 2025 Sep 26;88(9):2041-2052. doi: 10.1021/acs.jnatprod.5c00360. Epub 2025 Aug 31.
Thirteen new 3,4--tirucallane triterpenoids (, , ), along with one new 2,3--tirucallane triterpenoid (), one new 3,4--29-nor-tirucallane triterpenoid (), one new 3,4--28,29-dinor-tirucallane triterpenoid (), and a known tirucallane analogue () were isolated from leaves of These triterpenoids were structurally determined by spectroscopic methods, including HRESIMS, NMR spectroscopic analyses, single-crystal X-ray diffraction, and ECD data. The cytotoxicity evaluation revealed that compounds and exhibited moderate activity against HCT-116 and DLD-1 cell lines with IC values ranging from 3.7 to 4.4 μM. The Annexin V-PE/7-AAD staining assay indicated that both and induced apoptosis in a concentration-dependent manner. Further studies showed that compounds , , , and exhibited suppression effects on NO production in LPS-induced RAW 264.7 cells, with IC values ranging from 17.1 ± 3.3 to 49.8 ± 6.5 μM.
从[植物名称]的叶子中分离出13个新的3,4-齐墩果烷型三萜（[化合物1]、[化合物2]、……），以及1个新的2,3-齐墩果烷型三萜（[化合物14]）、1个新的3,4-29-降齐墩果烷型三萜（[化合物15]）、1个新的3,4-28,29-双降齐墩果烷型三萜（[化合物16]）和1个已知的齐墩果烷类似物（[化合物17]）。这些三萜通过高分辨电喷雾电离质谱（HRESIMS）、核磁共振光谱分析、单晶X射线衍射和电子圆二色光谱（ECD）数据等光谱方法进行结构测定。细胞毒性评估显示，化合物[化合物1]和[化合物2]对HCT-116和DLD-1细胞系表现出中等活性，IC50值在3.7至4.4 μM范围内。膜联蛋白V-异硫氰酸荧光素/7-氨基放线菌素D（Annexin V-PE/7-AAD）染色分析表明，[化合物1]和[化合物2]均以浓度依赖的方式诱导细胞凋亡。进一步研究表明，化合物[化合物3]、[化合物4]、[化合物5]和[化合物6]对脂多糖（LPS）诱导的RAW 264.7细胞中一氧化氮（NO）的产生具有抑制作用，IC50值在17.1±3.3至49.8±6.5 μM范围内。
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