Dehdashti Farshad, Shirkani Hossein, Mehrabi Mohsen, Ahmadi Amirhossein
Physics Department, Persian Gulf University, P. O. Box: 7516913817, Bushehr, Iran.
Department of Biological Science and Technology, Persian Gulf University, P. O. Box: 7516913817, Bushehr, Iran.
Sci Rep. 2025 Sep 2;15(1):32269. doi: 10.1038/s41598-025-17797-2.
In this research, biocompatible α-FeO nanoparticles were prepared as an agent for photothermal and photodynamic therapy methods by combining green synthesis and hydrothermal methods. The addition of chitosan bio-polymer played a crucial role in this process, as it not only stabilized the suspension of nanoparticles but also enhanced their biocompatibility. This stability was confirmed by zeta potential analysis Various analyses such as transmission electron microscope, X-ray diffraction, UV-visible spectrum, and Fourier transform infrared spectrum were performed to determine the structural and optical characteristics of the nanocomposite. The average size of the spherical crystals of α-FeO nanoparticles was estimated at 43 nm using the Williamson-Hall equation. The corresponding band gap value of nanoparticles was estimated at 1.8 eV by drawing a Tauc diagram. Photothermal effects for several different concentrations of an aqueous solution of CS-nanocomposite were measured by an 808 nm laser with a power density of 1 W/cm and the concentration of 5 mg/ml was chosen as the optimal concentration for use in photothermal therapy. The value of the photothermal conversion efficiency of this nanocomposite was determined at 7% using Roper's equation. To investigate the photodynamic properties of nanoparticles, a methylene blue probe was used to detect active oxygen species. Finally, an MTT assay studied the cytotoxicity of nanocomposite on AGS cells before and after laser irradiation. Under laser irradiation, cell viability at concentrations of 250ppm and 500ppm was 88% and 69%, respectively, compared to the control cells, confirming this nanocomposite's photothermal therapy and photodynamic therapy effects.
在本研究中，通过结合绿色合成法和水热法制备了具有生物相容性的α-FeO纳米颗粒，作为光热疗法和光动力疗法的药剂。壳聚糖生物聚合物的添加在这一过程中起着关键作用，因为它不仅稳定了纳米颗粒的悬浮液，还增强了它们的生物相容性。通过zeta电位分析证实了这种稳定性。进行了各种分析，如透射电子显微镜、X射线衍射、紫外可见光谱和傅里叶变换红外光谱分析，以确定纳米复合材料的结构和光学特性。使用威廉姆森-霍尔方程估计α-FeO纳米颗粒球形晶体的平均尺寸为43纳米。通过绘制陶克图估计纳米颗粒的相应带隙值为1.8电子伏特。用功率密度为1W/cm的808nm激光测量了几种不同浓度的CS-纳米复合材料水溶液的光热效应，并选择5mg/ml的浓度作为光热疗法的最佳使用浓度。使用罗珀方程确定该纳米复合材料的光热转换效率值为7%。为了研究纳米颗粒的光动力特性，使用亚甲基蓝探针检测活性氧物种。最后，通过MTT试验研究了激光照射前后纳米复合材料对AGS细胞的细胞毒性。与对照细胞相比，在激光照射下，250ppm和500ppm浓度下的细胞活力分别为88%和69%，证实了这种纳米复合材料的光热疗法和光动力疗法效果。
