Faculty of Dentistry, Thammasat University, Pathum Thani, 12120, Thailand.
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
BMC Oral Health. 2024 Nov 17;24(1):1399. doi: 10.1186/s12903-024-05186-6.
Patients receiving head and neck radiation are at high risk for radiation caries. This study aimed to evaluate the remineralizing effects of an experimental 1.1% NaF (5000 ppmF) toothpaste containing Sr/F-doped bioactive glass nanoparticles (BAG or B) on demineralized irradiated dentin.
Fluoride concentration and pH stability of materials upon mixing with water were assessed using a fluoride-specific electrode (n = 3) for up to 3 months. Elemental release of materials in water was determined using ICP-OES (n = 3). Fourteen extracted molars were irradiated with a cumulative dose of 70 Gy. Each tooth was sectioned into 4 specimens (n = 14/group), demineralized, and subjected to pH cycling for 14 days. Groups were treated with Prevident (PV), E5000, E5000B, and deionized water twice daily. Remineralization was assessed using ATR-FTIR (mineral-to-collagen ratio) (n = 14). Mineral precipitation was additionally examined with SEM-EDX. The in vitro cytotoxicity of the materials on L929 mouse fibrosarcoma was evaluated with the MTT test (n = 3). Kruskal-Wallis test, followed by Dunn's procedure, was used to compare the data between groups.
PV demonstrated greater pH and fluoride release stability than the experimental materials. E5000B exhibited a slight reduction of fluoride release (p < 0.01, R²=0.656) and an increase in pH with time (p = 0.006, R²=0.233). The highest increase in mineral-to-collagen ratio at 14 days was detected with PV (p < 0.05). E5000B also showed a significantly higher ratio than E5000 (p = 0.014). SEM-EDX detected mineral precipitation on dentin treated with PV and E5000B but not in E5000 and DI. The cell viability of PV (56%) was significantly lower than that of E5000 (94%) and E5000B (89%) (p < 0.05).
The use of 5000 ppm fluoride toothpaste enhanced the remineralization of irradiated demineralized dentin, highlighting a potentially valuable strategy for preventing radiation caries. Adding bioactive glass further promoted remineralization but may require formulation adjustments to maintain toothpaste stability for clinical use.
接受头颈部放射治疗的患者发生放射性龋齿的风险很高。本研究旨在评估含有 Sr/F 掺杂生物活性玻璃纳米颗粒（BAG 或 B）的实验性 1.1%NaF（5000ppmF）牙膏对脱矿辐射牙本质的再矿化效果。
使用氟化物专用电极（n=3）评估材料与水混合后的氟化物浓度和 pH 稳定性，最长可达 3 个月。使用 ICP-OES（n=3）测定材料在水中的元素释放情况。将 14 颗离体磨牙用累积剂量 70Gy 照射。每个牙齿被切成 4 个标本（n=14/组），脱矿，然后进行 14 天的 pH 循环。将 Prevident（PV）、E5000、E5000B 和去离子水每天两次处理组。使用 ATR-FTIR（矿物质与胶原蛋白的比率）（n=14）评估再矿化情况。使用 SEM-EDX 进一步检查矿物沉淀。使用 MTT 试验（n=3）评估材料对 L929 小鼠纤维肉瘤的体外细胞毒性。采用 Kruskal-Wallis 检验，然后采用 Dunn 程序对组间数据进行比较。
PV 显示出比实验材料更好的 pH 和氟化物释放稳定性。E5000B 显示出氟化物释放略有减少（p<0.01，R²=0.656）和随时间 pH 增加（p=0.006，R²=0.233）。在第 14 天，PV 检测到的矿物质与胶原蛋白的比率增加最高（p<0.05）。E5000B 与 E5000 相比，比率也明显更高（p=0.014）。SEM-EDX 检测到用 PV 和 E5000B 处理的牙本质上有矿物质沉淀，但在 E5000 和 DI 中没有。PV（56%）的细胞活力明显低于 E5000（94%）和 E5000B（89%）（p<0.05）。
使用 5000ppm 氟化物牙膏增强了辐射脱矿牙本质的再矿化，这突出了一种预防放射性龋齿的潜在有价值策略。添加生物活性玻璃进一步促进了再矿化，但可能需要调整配方以保持牙膏的稳定性，以便于临床应用。
