Lee Hanna, Gashi Denalda, Batool Syeda Maheen, Escobedo Ana K, Carter Bob S, Balaj Leonora
Department of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
bioRxiv. 2025 Aug 21:2025.08.15.670588. doi: 10.1101/2025.08.15.670588.
m6ASeqTools is an R package designed to streamline the post-processing and interpretation of site-level m6A predictions from m6Anet. It provides descriptive summaries of m6A distribution across transcripts, genes, biotypes, and transcript regions, and enables condition comparisons through a calculated weighted modification ratio. By integrating differential gene expression data, the package links methylation changes with expression differences, providing biotype-specific and region-specific insights into how m6A localization patterns relate to transcriptional regulation.
m6ASeqTools是一个R包,旨在简化对来自m6Anet的位点水平m6A预测结果的后处理和解读。它提供了m6A在转录本、基因、生物型和转录本区域分布的描述性总结,并通过计算加权修饰率实现条件比较。通过整合差异基因表达数据,该软件包将甲基化变化与表达差异联系起来,提供了生物型特异性和区域特异性的见解,以了解m6A定位模式与转录调控之间的关系。