用放射性铟标记粒细胞：细胞分离和标记参数

Labelling of granulocytes with radioindium: cell isolation and labelling parameters.

作者信息

Syrjälä M T

出版信息

Scand J Haematol. 1985 Nov;35(5):579-83. doi: 10.1111/j.1600-0609.1985.tb02831.x.

DOI:10.1111/j.1600-0609.1985.tb02831.x

Abstract

A method for isolation and labelling of purified granulocytes with 111In-oxine for scintigraphy of inflammatory processes is described. The procedure, on average, yielded 27.6% of the initial granulocytes in the sample with minimal lymphocyte and platelet contamination. The mean erythrocyte/granulocyte ratio was 0.95. In 20 experiments, 85.3% of the total radioactivity was attached to granulocytes, 8.7% to erythrocytes and 5.9% was in the plasma. The sensitivity and the specificity for the detection of acute inflammatory processes in 44 patients were 91.3% and 81.8%, respectively.

摘要

描述了一种用铟-111 奥克辛分离和标记纯化粒细胞以用于炎症过程闪烁显像的方法。该程序平均产生样本中初始粒细胞的 27.6%，淋巴细胞和血小板污染最小。平均红细胞/粒细胞比率为 0.95。在 20 次实验中，总放射性的 85.3%附着于粒细胞，8.7%附着于红细胞，5.9%存在于血浆中。44 例患者中检测急性炎症过程的敏感性和特异性分别为 91.3%和 81.8%。