Pfeiffer G, Erten J, Deubelbeiss K
Eur J Nucl Med. 1982;7(5):195-6. doi: 10.1007/BF00256461.
The isolation of granulocytes from whole blood and labelling with Indium 111-oxine sulphate are described in detail. To isolate the cells a two-step method was used: (1) removal of the red blood cells by methyl cellulose-Ronpacon and (2) separation of the leucocyte-rich plasma with a double gradient (1077, 1097) technique. 111In-oxine sulphate was prepared by adding 111In-chloride to a buffered solution of oxine sulphate. The labelling of the granulocytes with 111In-oxine sulphate was done by incubation at room temperature for 5 min.
本文详细描述了从全血中分离粒细胞并用硫酸铟-111-奥克辛进行标记的方法。为了分离细胞，采用了两步法：(1) 用甲基纤维素-罗恩帕康去除红细胞；(2) 用双梯度 (1077, 1097) 技术分离富含白细胞的血浆。硫酸铟-111-奥克辛是通过将氯化铟-111加入到硫酸奥克辛的缓冲溶液中制备的。用硫酸铟-111-奥克辛对粒细胞进行标记是在室温下孵育5分钟完成的。
