Kim Mi Hye, Jin Seong Chul, Yang Woong Mo
College of Korean Medicine, Woosuk University, Jeonju, Korea.
Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
Integr Med Res. 2025 Dec;14(4):101201. doi: 10.1016/j.imr.2025.101201. Epub 2025 Jul 14.
Excessive fat deposition in localized adiposity is known to induce severe medical diseases as well as aesthetic problems. Of late, LIPOSA-T pharmacopuncture, a new herbal pharmacopuncture consisting of the cortex of and bark of , is developed as a non-surgical injection for dissolving localized fat deposits.
The network pharmacology analysis was carried out with the target gene sets of constituents of and . Male C57BL/6 J mice were induced obesity and injected LIPOSA-T pharmacopuncture into inguinal fat pad. The fat weight and size were analyzed using dual energy X-ray absorptiometry.
The possible pathways and mechanism of action of LIPOSA-T were found to be mainly related to the fatty acid biosynthesis, glycolysis and glycogenesis in KEGG Pathways database. Subcutaneous injection of the LIPOSA-T pharmacopuncture significantly reduced the inguinal fat tissues weight and enlarged adipocyte size. In addition, the phosphorylated IRS with the PEPCK and G6p expressions were increased by the LIPOSA-T injection. Following the increase of AMPK expression, the fatty acid synthesis enzyme and lipolytic enzymes were regulated by the LIPOSA-T.
Taken together, LIPOSA-T exerted the catabolic effects on fat deposition in obesity by regulating the glucose production, lipid synthesis and TAG hydrolysis in consistent with the prediction results. Based on the findings, LIPOSA-T pharmacopuncture is expected to be a dissolving injection for localized fat.
已知局部肥胖中过多的脂肪沉积会引发严重的医学疾病以及美学问题。最近，LIPOSA-T 中药针剂作为一种用于溶解局部脂肪沉积的非手术注射剂被研发出来，它是一种由[植物 A]的皮层和[植物 B]的树皮组成的新型中药针剂。
对[植物 A]和[植物 B]成分的靶基因集进行网络药理学分析。将雄性 C57BL/6 J 小鼠诱导成肥胖模型，并将 LIPOSA-T 中药针剂注射到腹股沟脂肪垫中。使用双能 X 射线吸收法分析脂肪重量和大小。
在 KEGG 通路数据库中发现，LIPOSA-T 可能的作用途径和机制主要与脂肪酸生物合成、糖酵解和糖原生成有关。皮下注射 LIPOSA-T 中药针剂可显著降低腹股沟脂肪组织重量，并增大脂肪细胞大小。此外，LIPOSA-T 注射可使磷酸化的 IRS 以及磷酸烯醇式丙酮酸羧激酶（PEPCK）和葡萄糖-6-磷酸酶（G6p）的表达增加。随着腺苷酸活化蛋白激酶（AMPK）表达的增加，LIPOSA-T 对脂肪酸合成酶和脂解酶进行调节。
综上所述，LIPOSA-T 通过调节葡萄糖生成、脂质合成和甘油三酯水解，对肥胖中的脂肪沉积发挥分解代谢作用，这与预测结果一致。基于这些发现，LIPOSA-T 中药针剂有望成为一种用于局部脂肪溶解的注射剂。
