Tsankova K
Vet Med Nauki. 1977;14(1):47-53.
Mahadevan and Tatum's method was used to isolate four fractions from the bacterial walls of Brucella abortus 99. Determined were the amino acid composition and the purity (by means of the complement fixation test) of these fractions. Infrared spectroscopy was carried out as well. It was found that: 1. The particular fractions differ by their amino acid composition. 2. The fractions are practically pure. 3. The functional groups established confirm previous data of the author on the chemical composition of the wall, and give an idea about the distribution of these groups among the individual fractions. The fractions obtained prove to be the building ingredients of the Brucella abortus 99 bacterial wall.
采用马哈德万和塔特姆的方法从流产布鲁氏菌99的细胞壁中分离出四个组分。测定了这些组分的氨基酸组成和纯度(通过补体结合试验)。还进行了红外光谱分析。结果发现:1. 各个组分的氨基酸组成不同。2. 这些组分几乎是纯的。3. 所确定的官能团证实了作者先前关于细胞壁化学成分的数据,并让人了解这些基团在各个组分中的分布情况。所获得的组分被证明是流产布鲁氏菌99细胞壁的组成成分。