Tsankova K
Vet Med Nauki. 1975;12(10):35-9.
Cell walls were obtained from Brucella abortus 99. A peptidoglucan was isolated from them. Test animals were immunized (albino mice) with pure human gammaglobulin, and in a series of experiments the peptidoglucan was added to the antigen, while in another one it was injected 24 hours later. The titer of the separated serum was determined in terms of the amount of the complement-fixing antibodies present, using the microreaction of complement fixation. It was established that with the addition of the peptidoglucan to the antigen the titer of the newly produced antibodies rose several times. If injected later (24 hours) it did not affect the production of antibodies. Discussed is the mechanism substantiating the rise of the antibody level.
从流产布鲁氏菌99株中获取细胞壁。从这些细胞壁中分离出一种肽聚糖。用纯人丙种球蛋白对实验动物（白化小鼠）进行免疫，在一系列实验中，将肽聚糖添加到抗原中，而在另一组实验中，在24小时后注射肽聚糖。使用补体结合微量反应，根据存在的补体结合抗体的量来测定分离血清的效价。已确定，将肽聚糖添加到抗原中时，新产生抗体的效价会提高几倍。如果在后期（24小时）注射，则不会影响抗体的产生。文中讨论了支持抗体水平升高的机制。
