Forsberg Fredrik, Kjellström Sven, Petrlova Jitka, Puthia Manoj, Schmidtchen Artur, Malmström Johan, Hartman Erik
Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Lund, Sweden.
Division of Extracellular Signaling and Cell Fate, Department of Experimental Medical Sciences, Lund University, Lund, Sweden.
Sci Data. 2025 Sep 2;12(1):1533. doi: 10.1038/s41597-025-05842-8.
Recently, mass spectrometry based peptidomics studies have proven useful in the identification of biomarkers and bioactive peptide-based therapeutics. Here, we present a dataset comprised of temporal wound fluid peptidomics data from highly defined porcine models. Wound fluids from porcine wounds infected with Staphylococcus aureus and Pseudomonas aeruginosa, and uninfected controls, were sampled at different timepoints of the infection. Peptides were extracted from the samples, followed by liquid chromatography tandem mass spectrometry analysis in data dependent acquisition mode. The resulting spectra and searched files have been deposited to online repositories and made easily accessible to enable further investigations of the infected and uninfected wound fluid peptidome.
最近，基于质谱的肽组学研究已被证明在生物标志物鉴定和基于生物活性肽的治疗方面很有用。在此，我们展示了一个数据集，该数据集由来自高度明确的猪模型的创伤液肽组学数据组成。在感染的不同时间点采集了感染金黄色葡萄球菌和铜绿假单胞菌的猪伤口以及未感染对照的创伤液。从样品中提取肽，然后以数据依赖采集模式进行液相色谱串联质谱分析。所得光谱和搜索文件已存入在线数据库，便于获取，以进一步研究感染和未感染的创伤液肽组。
