Agrahari Raj Kishan, Aoki Daisuke, Fujiwara Toru, Kamiya Takehiro
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan.
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-Cho, Inage-Ku, Chiba-Shi, Chiba, 263-8522, Japan.
BMC Plant Biol. 2025 Sep 2;25(1):1182. doi: 10.1186/s12870-025-07248-5.
Sustainable agriculture faces growing challenges in boosting food production while minimizing environmental impact, highlighting the need for innovative solutions. The "plastics to fertilizers" concept, which converts poly(isosorbide carbonate) (PIC) derived from plastic waste into urea and isosorbide, presents a promising approach, as we have previously reported (Abe in Green Chem 23:9030-9037, 2021). While urea's role in plant nutrition is well established, the effect of isosorbide on plant growth and development remains largely unexplored. This study evaluates the impact of exogenous isosorbide treatment on Arabidopsis thaliana, aiming to unveil its potential as a biostimulant. Plants were grown in media with varying concentrations (0-10 mM) of isosorbide. Based on the optimal dose, determined by enhancements in shoot biomass and primary root length, we further analysed several parameters, including carbon and nitrogen content, carbon-to-nitrogen (C/N) ratio, nitrogen use efficiency (NUE), ionomic profiles, transcriptomic changes, and stress tolerance. Our results demonstrate that isosorbide treatment significantly promotes plant growth, with a significant increase in shoot biomass, improved C/N ratio, and enhanced NUE. Ionome analysis revealed altered distributions of essential elements in shoots and roots, indicating that isosorbide influence nutrient uptake and allocation. In addition, isosorbide enhanced plant growth under nitrogen deficiency and salt stress conditions. Transcriptomic analysis identified 447 differentially expressed genes in shoots and 327 in the roots, with significant enriched in pathways related to stress adaptation, metabolism, and hormonal regulation. Together, these findings provide novel insights into the biostimulant potential of isosorbide, highlighting its robust impact on plant growth and stress resilience while offering an innovative link between polymer recycling and sustainable agriculture.
可持续农业在提高粮食产量的同时将环境影响降至最低方面面临着越来越大的挑战，这凸显了对创新解决方案的需求。如我们之前所报道的（阿部，《绿色化学》23:9030 - 9037，2021年），“塑料转化为肥料”的概念，即将源自塑料垃圾的聚（异山梨醇碳酸酯）（PIC）转化为尿素和异山梨醇，是一种很有前景的方法。虽然尿素在植物营养中的作用已得到充分证实，但异山梨醇对植物生长发育的影响在很大程度上仍未得到探索。本研究评估了外源异山梨醇处理对拟南芥的影响，旨在揭示其作为生物刺激剂的潜力。将植物种植在含有不同浓度（0 - 10 mM）异山梨醇的培养基中。根据通过地上部生物量和初生根长度的增加所确定的最佳剂量，我们进一步分析了几个参数，包括碳和氮含量、碳氮比（C/N）、氮利用效率（NUE）、离子组图谱、转录组变化和胁迫耐受性。我们的结果表明，异山梨醇处理显著促进植物生长，地上部生物量显著增加，C/N比改善，NUE提高。离子组分析揭示了地上部和根部必需元素分布的改变，表明异山梨醇影响养分吸收和分配。此外，异山梨醇在缺氮和盐胁迫条件下增强了植物生长。转录组分析确定了地上部447个差异表达基因和根部327个差异表达基因，这些基因在与胁迫适应、代谢和激素调节相关的途径中显著富集。总之，这些发现为异山梨醇的生物刺激剂潜力提供了新的见解，突出了其对植物生长和胁迫恢复力的强大影响，同时在聚合物回收利用与可持续农业之间提供了一种创新联系。
