Yang Wenwu, Li Jinjin, Zhou You, Wang Yuhan, Wenting Wenting, Zhou Nong, Wu Qiang-Sheng
Chongqing Institute for Food and Drug Control, Chongqing, China.
Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing, China.
Plant Signal Behav. 2025 Dec 31;20(1):2554917. doi: 10.1080/15592324.2025.2554917. Epub 2025 Sep 3.
is a valuable traditional Chinese medicinal plant that is prone to germplasm degradation during long-term continuous monoculture. Allelopathic autotoxicity, which is mediated primarily by phenolic acids, is considered a major factor contributing to this degradation. To reveal the accumulation patterns of phenolic acids in the rhizospheric soil of under continuous monoculture, five phenolic acids (-hydroxybenzoic acid, vanillic acid, syringic acid, -coumaric acid, and ferulic acid) in the rhizospheric soil of across 1-5 y, and various fertilizer regimes (chemical fertilizer, chemical fertilizer + organic fertilizer, and organic fertilizer) were determined to assess their accumulation characteristics, along with soil fertility parameters. The result showed that the levels of available nitrogen, Olsen-phosphorus, and available potassium in chemical fertilizer and chemical fertilizer + organic fertilizer, along with the organic matter content in all three soil samples, showed a decreasing trend over time, while organic fertilizer exhibited significant fluctuations without a clear pattern. The phenolic acid content in the rhizospheric soil initially increased and then generally decreased in later stages. After 5 y of cultivation, the soils treated with organic fertilizer exhibited lower phenolic acid levels than those treated with chemical fertilizer. The accumulation patterns of individual phenolic acids varied with fertilizer type and cultivation period, with organic fertilizer showing the most consistent patterns across all phenolic acids. There was a positive correlation among the five phenolic acids, along with a significant positive correlation between soil organic matter and vanillic acid and ferulic acid. These findings suggest that long-term monoculture leads to distinct accumulation characteristics of phenolic acids in the rhizospheric soil of , and the application of organic fertilizer can mitigate such accumulation.
是一种珍贵的传统中药材，在长期连续单作过程中容易发生种质退化。化感自毒作用主要由酚酸介导，被认为是导致这种退化的主要因素。为揭示连续单作条件下该中药材根际土壤中酚酸的积累模式，测定了连续1 - 5年种植的该中药材根际土壤中的5种酚酸（对羟基苯甲酸、香草酸、丁香酸、对香豆酸和阿魏酸）以及不同施肥制度（化肥、化肥 + 有机肥和有机肥）下的酚酸积累特征，并测定了土壤肥力参数。结果表明，化肥和化肥 + 有机肥处理下的有效氮、 Olsen磷和有效钾含量，以及所有三种土壤样品中的有机质含量均随时间呈下降趋势，而有机肥处理的土壤有机质含量波动较大，无明显规律。根际土壤中酚酸含量最初增加，后期总体下降。种植5年后，有机肥处理的土壤中酚酸含量低于化肥处理的土壤。不同酚酸的积累模式随肥料类型和种植时期而变化，有机肥处理下所有酚酸的积累模式最为一致。5种酚酸之间呈正相关，土壤有机质与香草酸和阿魏酸之间呈显著正相关。这些研究结果表明，长期单作导致该中药材根际土壤中酚酸具有明显的积累特征，施用有机肥可减轻这种积累。
