Chen Xiao-Die, Sun Cheng-He, Chen Tai-Yu, Sun Zhen-Yu, Lu Chang-Hu
College of Life Sciences Nanjing Forestry University Nanjing China.
Ecol Evol. 2025 Sep 1;15(9):e72094. doi: 10.1002/ece3.72094. eCollection 2025 Sep.
Bats belong to the order Chiroptera, which represents the second most diverse order among mammals. Bats provide critical ecosystem services through mosquito population control, suppression of agricultural arthropod pests, pollination facilitation, and seed dispersal, while also contributing to human health preservation and economic well-being. Moreover, they have an essential function in the ecosystem of the Earth. However, the NCBI database contains 36 mitochondrial genomes of the genus Kaup, 1829, and additional data are necessary to conserve the species diversity. To elucidate the phylogenetic positions of (Horsfield, 1855) and Peters, 1870 within , high-throughput sequencing technology was employed in this study to obtain the mitochondrial genomes of these two species and to reconstruct a phylogenetic tree of this genus based on 13 protein-coding genes (PCGs). The mitochondrial genomes of and were determined to be 17,067 and 17,104 base pairs in length, respectively, including 22 transfer RNA genes, 13 PCGs, 2 ribosomal RNAs, and a D-loop. Base composition revealed a marked preference for A and T nucleotides, with the highest A + T content in the D-loop (65.6%). Using Bayesian inference and maximum likelihood methods to reconstruct the phylogenetic tree, the results indicated that is monophyletic, with early speciation events occurring within the group. , (Peters, 1869) and formed one clade, with and exhibiting a closer phylogenetic relationship with each other than with . Our analytical data enhance the foundational database of mitochondrial genomes, enhancing our understanding of the evolutionary relationships among species within this genus. Highlighting the evolutionary relationships among different species of provides a solid foundation for subsequent studies on the adaptive evolution and selective pressure in bats.
蝙蝠属于翼手目，是哺乳动物中第二大多样化的目。蝙蝠通过控制蚊子数量、抑制农业节肢动物害虫、促进授粉和传播种子，提供关键的生态系统服务，同时也有助于人类健康保护和经济福祉。此外，它们在地球生态系统中具有重要作用。然而，NCBI数据库包含1829年考普属的36个线粒体基因组，需要更多数据来保护物种多样性。为了阐明1855年霍斯菲尔德的（某种蝙蝠）和1870年彼得斯的（某种蝙蝠）在考普属中的系统发育位置，本研究采用高通量测序技术获得这两个物种的线粒体基因组，并基于13个蛋白质编码基因（PCGs）重建该属的系统发育树。经测定，（某种蝙蝠）和（另一种蝙蝠）的线粒体基因组长度分别为17,067和17,104个碱基对，包括22个转移RNA基因、13个PCGs、2个核糖体RNA和一个D环。碱基组成显示对A和T核苷酸有明显偏好，D环中的A+T含量最高（65.6%）。使用贝叶斯推断和最大似然法重建系统发育树，结果表明（考普属）是单系的，早期物种形成事件发生在该类群内。（某种蝙蝠）、1869年彼得斯的（某种蝙蝠）和（另一种蝙蝠）形成一个分支，（某种蝙蝠）和（另一种蝙蝠）之间的系统发育关系比它们与（另一种蝙蝠）的关系更密切。我们的分析数据增强了考普属线粒体基因组的基础数据库，加深了我们对该属内物种进化关系的理解。突出不同考普属物种之间的进化关系为后续蝙蝠适应性进化和选择压力的研究提供了坚实基础。
