Zhu Qinghua, Ma Yixuan, Zhang Tong, Liu Weirong, Zhang Songbai, Chen Yue, Peng Di, Zhang Xin
MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River, Yangtze University, Jingzhou 434025, China.
Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Biology (Basel). 2025 Jul 23;14(8):921. doi: 10.3390/biology14080921.
The cultivation of cowpea (), a vital vegetable crop, faces significant threats from spp.-induced root rot. In this study, three fungal pathogens ( HKFf, HKFi, and HKFo) were isolated from symptomatic cowpea plants, and we screened 90 rhizobacteria from healthy rhizospheres using six culture media. Among these pathogens, TSA-10E showed a notable suppression of HKFo (63.21%), HKFi (55.16%), and HKFf (50.93%). In addition, KB-6 inhibited the mycelial growth of HKFi and HKFo by 42.39% and 47.93%, respectively. Critically, cell-free filtrates from TSA-10E and KB-6 cultures reduced conidial germination in HKFo and HKFi, highlighting their role in disrupting the early infection stages. In greenhouse trials, TSA-10E and KB-6 reduced disease severity by 48.7% and 40.4%, respectively, with treated plants maintaining healthy growth while untreated controls succumbed to wilting. Broad-spectrum assays revealed that TSA-6E and TSA-10E were potent antagonists against both economic and grain crop pathogens. These findings underscore the potential of rhizobacteria as sustainable biocontrol agents for managing root rot disease caused by spp. in cowpea cultivation.
豇豆是一种重要的蔬菜作物，其种植面临着由[病原菌名称未给出]引起的根腐病的重大威胁。在本研究中，从有症状的豇豆植株中分离出三种真菌病原体（HKFf、HKFi和HKFo），并使用六种培养基从健康根际筛选了90种根际细菌。在这些病原体中，TSA - 10E对HKFo（63.21%）、HKFi（55.16%）和HKFf（50.93%）表现出显著的抑制作用。此外，KB - 6分别抑制HKFi和HKFo的菌丝生长42.39%和47.93%。至关重要的是，TSA - 10E和KB - 6培养物的无细胞滤液降低了HKFo和HKFi中的分生孢子萌发，突出了它们在破坏早期感染阶段的作用。在温室试验中，TSA - 10E和KB - 6分别将病害严重程度降低了48.7%和40.4%，处理过的植株保持健康生长，而未处理的对照则枯萎死亡。广谱试验表明，TSA - 6E和TSA - 10E对经济作物和谷物作物病原体都是有效的拮抗剂。这些发现强调了根际细菌作为可持续生物防治剂用于管理豇豆种植中由[病原菌名称未给出]引起的根腐病的潜力。
