使用市售高锰酸钾和葡萄糖氧化酶对葡萄糖进行双检测:比色法和基于吸光度的方法。

Dual Detection of Glucose Using Commercial Potassium Permanganate and Glucose Oxidase: Colorimetric and Absorbance-Based Approaches.

作者信息

Hoang Chau Nguyen Minh, Nguyen Son Hai, Tran Mai Thi

机构信息

VinUni-Illinois Smart Health Center, VinUniversity, Hanoi 100000, Vietnam.

School of Mechanical Engineering, Hanoi University of Science and Technology, Hanoi 100000, Vietnam.

出版信息

ACS Omega. 2025 Aug 15;10(33):37514-37523. doi: 10.1021/acsomega.5c03564. eCollection 2025 Aug 26.

Abstract

Accurate and accessible glucose detection is essential for clinical diagnostics, point-of-care testing, food safety, and biosensing applications. In this study, we present a simple, scalable, and dual-mode glucose sensor that integrates commercial potassium permanganate (KMnO) with glucose oxidase to enable sensitive and selective detection in the clinically critical range of 1-5 mM. Leveraging the strong oxidative power and distinct optical characteristics of KMnO, the sensor operates via both absorbance measurement at 400 nm and visual colorimetric analysis, displaying a clear color change from purple to pink and yellow upon reaction with glucose. The system achieves a low detection limit of 0.63 mM and demonstrates high specificity, effectively minimizing interference from substances such as maltose, sucrose, galactose, citric acid, thiourea, and uric acid. Recovery rates ranging from 84.8% to 104.8% in glucose-spiked solutions and up to 99.6% in artificial saliva validate its reliability and practical applicability. By combining colorimetric simplicity with quantitative absorbance measurements, this platform provides an efficient, eco-friendly, and cost-effective solution for glucose monitoring with strong potential for integration into disposable test strips, portable devices, and wearable biosensors for real-time health tracking.

摘要

准确且易于使用的葡萄糖检测对于临床诊断、即时检测、食品安全和生物传感应用至关重要。在本研究中,我们展示了一种简单、可扩展的双模式葡萄糖传感器,该传感器将商业高锰酸钾(KMnO)与葡萄糖氧化酶集成在一起,能够在1-5 mM这一临床关键范围内进行灵敏且选择性的检测。利用KMnO的强氧化能力和独特的光学特性,该传感器通过在400 nm处的吸光度测量和视觉比色分析进行操作,与葡萄糖反应时显示出从紫色到粉色再到黄色的明显颜色变化。该系统实现了0.63 mM的低检测限,并表现出高特异性,有效减少了麦芽糖、蔗糖、半乳糖、柠檬酸、硫脲和尿酸等物质的干扰。在加标葡萄糖溶液中的回收率为84.8%至104.8%,在人工唾液中的回收率高达99.6%,验证了其可靠性和实际适用性。通过将比色法的简便性与定量吸光度测量相结合,该平台为葡萄糖监测提供了一种高效、环保且经济高效的解决方案,具有很强的潜力集成到一次性测试条、便携式设备和可穿戴生物传感器中,用于实时健康跟踪。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0527/12406281/dd4caf517dad/ao5c03564_0001.jpg

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