Karasawa T, Shikata T, Abe K, Kanayama S, Noro M, Oda T
Acta Pathol Jpn. 1985 Nov;35(6):1333-42. doi: 10.1111/j.1440-1827.1985.tb01432.x.
An electron-microscopic study was carried out using chimpanzees' livers infected with experimental hepatitis B for the elucidation of intracellular development of HBV-associated ultrastructures and extracellular release of HBV. Core particles were first detected in the nucleus of liver cells at around the time of the first seropositiveness for HBsAg, and then in the cytoplasm. Subsequently, their budding into endoplasmic reticular cisterna was seen together with other core particles in the surrounding cytoplasm. Dane-like particles were seen in the cisterna, and also extracellularly nearby a liver cell with a marked proliferation of microvilli at the onset of liver cell injury. Thereafter, core-like particles were seen within electrondense amorphous material at the site of the contact between liver cell and lymphocyte. The above sequence of features suggested us the assembly of core particles and surface envelope at the cisternal membrane of endoplasmic reticulum, and a reversed pinocytosis whereby Dane or HBV particles were released extracellularly. The filamentous structures within endoplasmic reticular cisternae, which were thought to be HBsAg, were never detected.
为了阐明乙肝病毒相关超微结构的细胞内发育过程以及乙肝病毒的细胞外释放，我们对感染实验性乙型肝炎的黑猩猩肝脏进行了电子显微镜研究。核心颗粒首先在肝细胞的细胞核中被检测到，时间大约在首次出现乙肝表面抗原血清阳性的时候，随后在细胞质中也能看到。随后，它们出芽进入内质网池，周围细胞质中还有其他核心颗粒。在池中可以看到类 Dane 颗粒，在肝细胞损伤开始时，在微绒毛明显增生的肝细胞附近的细胞外也能看到。此后，在肝细胞与淋巴细胞接触部位的电子致密无定形物质中可以看到类核心颗粒。上述一系列特征提示我们，核心颗粒和表面包膜在内质网池膜上组装，并通过反向胞饮作用使 Dane 颗粒或乙肝病毒颗粒释放到细胞外。在内质网池中从未检测到被认为是乙肝表面抗原的丝状结构。
