Kamimura T, Yoshikawa A, Ichida F, Sasaki H
Hepatology. 1981 Sep-Oct;1(5):392-7. doi: 10.1002/hep.1840010504.
Electron microscopic observations in 30 cases of HBsAg positive liver disease and 12 asymptomatic carriers of HBsAg suggested the following mechanism of intracellular development of Dane particles: core particles migrated from the nucleus into the cytoplasm through the nuclear pores. Intracytoplasmic core particles protruded into the cisternae of endoplasmic reticulum by budding the outer coat of Dane particles being derived from the membrane of endoplasmic reticulum. Release of Dane particles into the blood stream by reversed pinocytosis was suggested by the finding of submembranous localization of endoplasmic reticulum containing these particles. No budding from the cell surface of the hepatocytes was encountered. Dane particles in the hepatocytes were detected in 14 of 15 cases positive for serum HBeAg while no particles were seen in 27 HBeAg negative cases, thus suggesting that serum HBeAg reflected ongoing replication of hepatitis B virus in the hepatocytes.
对30例乙肝表面抗原（HBsAg）阳性肝病患者及12例无症状HBsAg携带者进行的电子显微镜观察提示了Dane颗粒在细胞内的形成机制如下：核心颗粒通过核孔从细胞核迁移至细胞质。细胞质内的核心颗粒通过出芽方式突出到内质网池，Dane颗粒的外衣源自内质网膜。在内质网含有这些颗粒的亚膜定位的发现提示，Dane颗粒通过反向胞饮作用释放到血流中。未观察到从肝细胞表面出芽的情况。在15例血清HBeAg阳性病例中的14例肝细胞内检测到Dane颗粒，而在27例HBeAg阴性病例中未见到颗粒，因此提示血清HBeAg反映了乙肝病毒在肝细胞内正在进行的复制。
