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丙酮丁醇梭菌耐丁醇突变体的分离与鉴定

Isolation and characterization of butanol-resistant mutants of Clostridium acetobutylicum.

作者信息

Hermann M, Fayolle F, Marchal R, Podvin L, Sebald M, Vandecasteele J P

出版信息

Appl Environ Microbiol. 1985 Nov;50(5):1238-43. doi: 10.1128/aem.50.5.1238-1243.1985.

DOI:10.1128/aem.50.5.1238-1243.1985
PMID:4091556
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238732/
Abstract

In a wild-type strain of Clostridium acetobutylicum isolated from soil, solvent production appeared limited by butanol toxicity. Butanol-resistant mutants have been obtained which produced significantly higher solvent concentrations (about 30%) than the wild-type strain. Some other physiological differences were observed between a selected resistant mutant and the wild-type strain at the level of solvent resistance and sporulation.

摘要

从土壤中分离出的野生型丙酮丁醇梭菌菌株中，溶剂生产似乎受到丁醇毒性的限制。已经获得了抗丁醇突变体，其产生的溶剂浓度比野生型菌株显著更高（约30%）。在溶剂抗性和孢子形成水平上，观察到选定的抗性突变体与野生型菌株之间存在一些其他生理差异。

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本文引用的文献

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Studies on the acetone-butyl alcohol fermentation: Nutritional and other factors involved in the preparation of active suspensions of Cl. acetobutylicum (Weizmann).丙酮-丁醇发酵研究：与丙酮丁醇梭菌（魏茨曼氏菌）活性悬浮液制备相关的营养及其他因素
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Butanol Production by a Butanol-Tolerant Strain of Clostridium acetobutylicum in Extruded Corn Broth.在膨化玉米浆中利用耐丁醇的丙酮丁醇梭菌生产丁醇。
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