Liu Hai, Liu Songlang, Liao Liqiong, Liao Hongxing, Pan Weilun, Hu Jun
Department of Sports Medicine, Meizhou People's Hospital, Meizhou Academy of Medical Sciences, Meizhou, Guangdong, 514031, China.
Biomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong, 510515, China.
Mater Today Bio. 2025 Aug 26;34:102243. doi: 10.1016/j.mtbio.2025.102243. eCollection 2025 Oct.
Combining disinfection and bone regeneration in a one-step treatment is of significant clinical importance for chronic osteomyelitis, yet it remains a considerable challenge. To address this, we developed a dual stimulus-responsive decellularized extracellular matrix (dECM) cryogel (GC-dECM@CPN). The cryogel is composed of methacrylate gelatin (GelMA), carboxymethyl chitosan (CMCS), dECM, and temperature-sensitive phase-transition copper peroxide nanoparticles (CPNs). During the infection phase of chronic osteomyelitis, the cryogel exhibits a strong photothermal conversion efficiency under near-infrared (NIR) light irradiation, triggering the release of CPNs to perform chemodynamic therapy (CDT) in the acidic, infected environment. This process enhances macrophage polarization toward the M1 phenotype, promoting the phagocytosis of residual bacteria. Following bacterial eradication, the dECM within the cryogel facilitates the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts while reprogramming macrophages into the M2 phenotype, thereby establishing a conducive environment for bone regeneration. The GC-dECM@CPN cryogel exhibited desirable therapeutic efficacy in both and studies, with critical stages of chronic osteomyelitis-including early infection, initial bone regeneration, and recovery-investigated and discussed in detail. This study introduces an innovative dual stimulus-responsive dECM cryogel that effectively treats chronic osteomyelitis through a one-step surgical approach, offering valuable insights for clinical therapeutic strategies in this field.
在一步治疗中结合消毒和骨再生对慢性骨髓炎具有重要的临床意义，但仍然是一个相当大的挑战。为了解决这个问题，我们开发了一种双刺激响应性脱细胞细胞外基质（dECM）冷冻凝胶（GC-dECM@CPN）。该冷冻凝胶由甲基丙烯酸明胶（GelMA）、羧甲基壳聚糖（CMCS）、dECM和温度敏感相变过氧化铜纳米颗粒（CPN）组成。在慢性骨髓炎的感染阶段，该冷冻凝胶在近红外（NIR）光照射下表现出很强的光热转换效率，触发CPN的释放，在酸性感染环境中进行化学动力疗法（CDT）。这一过程增强巨噬细胞向M1表型的极化，促进残余细菌的吞噬作用。在细菌根除后，冷冻凝胶中的dECM促进骨髓间充质干细胞（BMSC）向成骨细胞分化，同时将巨噬细胞重编程为M2表型，从而为骨再生建立有利环境。GC-dECM@CPN冷冻凝胶在体内和体外研究中均表现出理想的治疗效果，并对慢性骨髓炎的关键阶段——包括早期感染、初始骨再生和恢复——进行了详细研究和讨论。本研究介绍了一种创新的双刺激响应性dECM冷冻凝胶，通过一步手术方法有效治疗慢性骨髓炎，为该领域的临床治疗策略提供了有价值的见解。
