Grove D I, Northern C, Dawkins H J
Aust J Exp Biol Med Sci. 1985 Oct;63 ( Pt 5):521-9. doi: 10.1038/icb.1985.56.
When Strongyloides ratti free-living infective larvae were incubated with peritoneal exudate cells from normal or previously infected mice, cell attachment occurred only in the presence of normal (NMS) or immune mouse serum (IMS). This non-specific effect was transitory with larvae being alive and free of cells 24 h after incubation. Cell attachment was mediated by complement. When incubated with infective larvae which had penetrated mouse skin, both normal and immune cells attached to larvae in the absence of serum. This effect was again transitory except when immune cells or immune serum were present, indicating a specific immunological mechanism. Again, larvae remained viable. When incubated with isolated parasitic adult worms, persistent cell attachment occurred in the presence of immune serum, immune cells or both, but all worms remained viable. This system provides a means for investigating the mechanisms of resistance to reinfection in strongyloidiasis.
将来自正常或先前感染小鼠的腹膜渗出细胞与鼠类圆线虫自由生活的感染性幼虫一起孵育时，只有在存在正常（NMS）或免疫小鼠血清（IMS）的情况下才会发生细胞附着。这种非特异性效应是短暂的，孵育24小时后幼虫存活且无细胞附着。细胞附着由补体介导。当与穿透小鼠皮肤的感染性幼虫一起孵育时，在无血清的情况下正常细胞和免疫细胞都会附着于幼虫。除了存在免疫细胞或免疫血清时，这种效应同样是短暂的，表明存在一种特异性免疫机制。同样，幼虫仍保持活力。当与分离出的寄生成虫一起孵育时，在存在免疫血清、免疫细胞或两者的情况下会发生持续的细胞附着，但所有蠕虫仍保持活力。该系统为研究类圆线虫病中抵抗再感染的机制提供了一种方法。
