Khaleque Md Abdul, Sazza Moumita Rahman, Akter Selina, Ali Md Romzan, Hossain Syed Imdadul, Saidur Rahman, Aly Saad Aly Mohamed, Khan Md Zaved H
Department of Chemical Engineering, Jashore University of Science and Technology Jashore 7408 Bangladesh
Laboratory of Nano-bio and Advanced Materials Engineering (NAME), Jashore University of Science and Technology Jashore 7408 Bangladesh.
RSC Adv. 2025 Sep 5;15(39):32041-32055. doi: 10.1039/d5ra03642a.
Bacterial detection is crucial for accurate clinical diagnostics and effective environmental monitoring. Particularly, , a pathogenic bacterium, can cause a wide range of infections, including meningitis, bloodstream infections, pneumonia, urinary tract infections, and wound or surgical site infections. Herein, a polypyrrole (PPy) functionalized TiCT -tin dioxide nanoparticle (SnO NPs) nanocomposite-based hybrid capacitive electrode for the electrochemical detection of ATCC 700603 is developed. The PPy layer was coated onto the TiCT -SnO NPs drop-casting, followed by immobilization of bacteriophages through a potentiostatic, charge-directed chronoamperometric approach. The resulting TiCT -SnO NPs/PPy/phage biosensor exhibited a wide dynamic detection range of 10 to 10 CFU mL, with excellent linearity confirmed by differential pulse voltammetry and electrochemical impedance spectroscopy. The nanocomposite was characterized by using a suite of techniques including FTIR, XRD, elemental mapping, cyclic voltammetry, and galvanostatic charge-discharge to understand its composition, structure, and electrochemical properties. The developed TiCT -SnO NPs/PPy hybrid electrode demonstrated typical supercapacitor behavior with a specific capacitance of 806.67 F g at 2.0 A g of current density, and exhibited exceptional cycling stability, storing 98.3% of its capacitance after 10 consecutive cycles. The selectivity of the modified electrode to detect while minimizing interference from various bacterial cells was assessed, exhibiting remarkable resilience, and remaining unaffected. Additionally, after eleven successive weeks of storage, the proposed sensor showed no discernible reduction current (∼98.1%), demonstrating an excellent stability. Despite the presence of background bacterial interference in the environmental sample, detection remained highly reliable and consistent with recovery efficiency ranging from 99.75 to 99.90%.
细菌检测对于准确的临床诊断和有效的环境监测至关重要。特别是，[具体细菌名称未给出]这种病原菌可导致多种感染，包括脑膜炎、血流感染、肺炎、尿路感染以及伤口或手术部位感染。在此，开发了一种基于聚吡咯（PPy）功能化的TiCT - 二氧化锡纳米颗粒（SnO NPs）纳米复合材料的混合电容电极，用于电化学检测ATCC 700603。通过滴铸法将PPy层涂覆在TiCT - SnO NPs上，然后通过恒电位、电荷导向计时电流法固定噬菌体。所得的TiCT - SnO NPs/PPy/噬菌体生物传感器展现出10至10 CFU/mL的宽动态检测范围，通过差分脉冲伏安法和电化学阻抗谱证实具有出色的线性。使用包括傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、元素映射、循环伏安法和恒电流充放电等一系列技术对纳米复合材料进行表征，以了解其组成、结构和电化学性质。所开发的TiCT - SnO NPs/PPy混合电极在2.0 A/g的电流密度下表现出典型的超级电容器行为，比电容为806.67 F/g，并展现出卓越的循环稳定性，在连续10个循环后仍保留其电容的98.3%。评估了修饰电极在检测[具体细菌名称未给出]时的选择性，同时将来自各种细菌细胞的干扰降至最低，显示出显著的抗干扰能力且不受影响。此外，在连续储存十一周后，所提出的传感器显示出无明显的电流降低（约98.1%），证明具有出色的稳定性。尽管环境样品中存在背景细菌干扰，但[具体细菌名称未给出]的检测仍然高度可靠且一致，回收率范围为99.75%至99.90%。
