Dsouza Venessa, Thoidingjam Goldee, Saleh Abdelrahman, Zavrel Michael
Technical University of Munich, Germany, TUM Campus Straubing for Biotechnology and Sustainability, Bioprocess Engineering, Uferstraße 53, D-94315 Straubing, Germany.
Munich Institute of Integrated Materials, Energy and Process Engineering, Technical University of Munich, Garching, Germany.
Biotechnol Rep (Amst). 2025 Aug 15;47:e00914. doi: 10.1016/j.btre.2025.e00914. eCollection 2025 Sep.
Exopolysaccharides are biopolymers with wide-ranging industrial applications. To substitute fossil-based by bio-based, biodegradable polymers, exopolysaccharide production needs to become much more efficient. Pullulan, produced by , is popular for its unique properties like film-formation, adhesiveness, biodegradability, etc. This study aims at demonstrating the optimization strategy on pullulan production using statistical optimization iteratively. The process followed a systematic approach including One Factor at A Time (OFAT) and Design Of Experiments (DOE). OFAT confirmed sucrose as carbon source, pH 6 and 4 days of incubation were optimum. Iterative Plackett-Burman Design (PBD) and Response Surface Methodology (RSM) further demonstrated interactions between significant media components and incubation conditions. The final pullulan titer was increased 6.34-fold to 113.5 ± 3.5 g/L of EPS, the highest titer achieved for this strain in shake flasks. Future investigations into fed-batch strategies and scale-up studies can be explored to further improve titers and reduce overall production costs.
胞外多糖是具有广泛工业应用的生物聚合物。为了用生物基、可生物降解的聚合物替代化石基聚合物，胞外多糖的生产需要变得更加高效。由[具体生产菌株]生产的普鲁兰多糖因其成膜、粘附性、生物降解性等独特性能而受到欢迎。本研究旨在通过迭代统计优化来展示普鲁兰多糖生产的优化策略。该过程采用了系统的方法，包括一次一因子法（OFAT）和实验设计（DOE）。OFAT确定蔗糖为碳源、pH值为6以及培养4天为最佳条件。迭代的Plackett-Burman设计（PBD）和响应面方法（RSM）进一步证明了重要培养基成分与培养条件之间的相互作用。最终的普鲁兰多糖滴度提高了6.34倍，达到113.5±3.5 g/L的胞外多糖，这是该菌株在摇瓶中获得的最高滴度。未来可以探索补料分批策略和放大研究，以进一步提高滴度并降低总体生产成本。
