Liu Bin, Otoi Takeshige, Namula Zhao, Widodo Oky Setyo, Hirata Maki, Nakai Aya, Lin Qingyi, Nakayama Yuichiro, Nagahara Megumi, Tanihara Fuminori
Bio-Innovation Research Center, Tokushima University, 779-3233 Tokushima, Japan.
Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, 60115 Surabaya, Indonesia.
Vet World. 2025 Jul;18(7):2078-2086. doi: 10.14202/vetworld.2025.2078-2086. Epub 2025 Jul 27.
Porcine follicular fluid (pFF) is frequently used to mimic the follicular microenvironment during maturation (IVM) of oocytes. However, the influence of oxidative stress levels within pFF on oocyte quality and embryo development remains unclear. This study aimed to investigate how varying oxidative stress index (OSI) of pFF affect porcine oocyte meiotic progression, fertilization, and embryonic development during IVM.
Oocytes were matured in IVM media supplemented with 30% pFF classified into low (OSI 19), medium (OSI 22), and high (OSI 25) oxidative stress groups, based on the ratio of diacron-reactive oxygen metabolites to biological antioxidant potential. Post-IVM, oocytes were assessed for meiotic stage, DNA fragmentation, reactive oxygen species (ROS), and glutathione (GSH) levels. Fertilization and embryo development outcomes were monitored following fertilization and culture.
The OSI 19 group showed significantly higher maturation to the metaphase II stage and improved fertilization and blastocyst formation rates compared to OSI 22 and OSI 25 groups (p < 0.05). ROS and GSH levels were also significantly elevated in OSI 19 oocytes, without an increase in DNA fragmentation. Blastocysts from the OSI 25 group exhibited significantly higher DNA fragmentation index than those from the OSI 19 group (p < 0.05).
The OSI of pFF modulates porcine oocyte competence and embryonic outcomes. Lower OSI is associated with enhanced antioxidant balance, meiotic maturation, and embryo quality. Monitoring pFF oxidative status may improve assisted reproductive outcomes in swine.
猪卵泡液（pFF）常用于模拟卵母细胞成熟（IVM）过程中的卵泡微环境。然而，pFF内氧化应激水平对卵母细胞质量和胚胎发育的影响仍不清楚。本研究旨在探讨pFF不同的氧化应激指数（OSI）如何影响猪卵母细胞在IVM期间的减数分裂进程、受精及胚胎发育。
卵母细胞在添加30% pFF的IVM培养基中成熟，根据二丙烯基活性氧代谢产物与生物抗氧化潜能的比值，将pFF分为低（OSI 19）、中（OSI 22）、高（OSI 25）氧化应激组。IVM后，评估卵母细胞的减数分裂阶段、DNA片段化、活性氧（ROS）和谷胱甘肽（GSH）水平。受精和培养后监测受精和胚胎发育结果。
与OSI 22和OSI 25组相比，OSI 19组的中期II期成熟率显著更高，受精和囊胚形成率也有所提高（p < 0.05）。OSI 19的卵母细胞中ROS和GSH水平也显著升高，DNA片段化未增加。OSI 25组的囊胚DNA片段化指数显著高于OSI 19组（p < 0.05）。
pFF的OSI调节猪卵母细胞的能力和胚胎发育结果。较低的OSI与增强的抗氧化平衡、减数分裂成熟和胚胎质量相关。监测pFF的氧化状态可能改善猪的辅助生殖结果。
