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小鼠浆细胞瘤中免疫球蛋白增强子缺失的特征分析。

Characterization of immunoglobulin enhancer deletions in murine plasmacytomas.

作者信息

Aguilera R J, Hope T J, Sakano H

出版信息

EMBO J. 1985 Dec 30;4(13B):3689-93. doi: 10.1002/j.1460-2075.1985.tb04136.x.

DOI:10.1002/j.1460-2075.1985.tb04136.x
PMID:4092693
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554719/
Abstract

We have analyzed enhancer deletions found in murine plasmacytomas by DNA cloning. This analysis revealed that the deletions occurred between the JH region and the switch region, removing the Ig heavy chain enhancer. The loss of the enhancer did not significantly affect the level of heavy chain expression as determined by RNA blots. Nucleotide sequence analysis revealed that there are no characteristic or homologous sequences around the recombination site. Extra nucleotides were found at the recombination sites, in a manner analogous to Ig and T-cell receptor V-D-J joining. The germline JH and switch sequences involved in the deletion were analyzed by the in vitro DNA cleavage system with an endonucleolytic activity purified from mouse fetal liver nuclear extracts. It was found that the germline JH DNA was strongly cleaved at the deletion recombination site.

摘要

我们通过DNA克隆分析了在鼠浆细胞瘤中发现的增强子缺失情况。该分析表明，缺失发生在JH区域和转换区域之间，移除了Ig重链增强子。如RNA印迹分析所示，增强子的缺失并未显著影响重链表达水平。核苷酸序列分析显示，重组位点周围没有特征性或同源序列。在重组位点发现了额外的核苷酸，其方式类似于Ig和T细胞受体V-D-J连接。通过从鼠胎儿肝核提取物中纯化的具有内切核酸酶活性的体外DNA切割系统，分析了参与缺失的种系JH和转换序列。结果发现，种系JH DNA在缺失重组位点被强烈切割。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a11/554719/19667a49caaf/emboj00279-0055-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a11/554719/171b100cf268/emboj00279-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a11/554719/5bda83111725/emboj00279-0055-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a11/554719/f173d6d8ffcc/emboj00279-0055-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a11/554719/19667a49caaf/emboj00279-0055-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a11/554719/171b100cf268/emboj00279-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a11/554719/5bda83111725/emboj00279-0055-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a11/554719/f173d6d8ffcc/emboj00279-0055-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a11/554719/19667a49caaf/emboj00279-0055-c.jpg

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Characterization of immunoglobulin enhancer deletions in murine plasmacytomas.小鼠浆细胞瘤中免疫球蛋白增强子缺失的特征分析。
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2
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Transposition of the immunoglobulin heavy chain enhancer to the myc oncogene in a murine plasmacytoma.在一个鼠浆细胞瘤中免疫球蛋白重链增强子易位至myc癌基因。
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Illegitimate recombination generates a class switch from C mu to C delta in an IgD-secreting plasmacytoma.在一个分泌IgD的浆细胞瘤中，非法重组导致了从Cμ到Cδ的类别转换。
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Deletions are associated with somatic rearrangement of immunoglobulin heavy chain genes.缺失与免疫球蛋白重链基因的体细胞重排相关。
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Novel myc oncogene RNA from abortive immunoglobulin-gene recombination in mouse plasmacytomas.来自小鼠浆细胞瘤中流产性免疫球蛋白基因重排的新型myc癌基因RNA
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DNA sequences near the site of reciprocal recombination between a c-myc oncogene and an immunoglobulin switch region.c-myc癌基因与免疫球蛋白转换区之间相互重组位点附近的DNA序列。
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7269-73. doi: 10.1073/pnas.80.23.7269.
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Unusual immunoglobulin DNA sequences from the nonexpressed chromosome of mouse normal B lymphocytes: implications for allelic exclusion and the DNA rearrangement process.来自小鼠正常B淋巴细胞非表达染色体的异常免疫球蛋白DNA序列：对等位基因排斥和DNA重排过程的影响。
J Immunol. 1987 Sep 1;139(5):1718-26.

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本文引用的文献

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Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells.转染至淋巴细胞中的免疫球蛋白重链基因的表达与调控
EMBO J. 1983;2(8):1373-8. doi: 10.1002/j.1460-2075.1983.tb01594.x.
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Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.细胞质斑点杂交。对多个小细胞或组织样本中相对mRNA水平的简单分析。
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Immunoglobulin gene transcription ceases upon deletion of a distant enhancer.免疫球蛋白基因转录在一个远距离增强子缺失后停止。
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Proc Natl Acad Sci U S A. 1981 Dec;78(12):7684-8. doi: 10.1073/pnas.78.12.7684.
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Switch region of immunoglobulin Cmu gene is composed of simple tandem repetitive sequences.免疫球蛋白Cμ基因的转换区由简单串联重复序列组成。
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Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes.免疫球蛋白重链基因多样性DNA片段（D）的鉴定及核苷酸序列
Nature. 1981 Apr 16;290(5807):562-5. doi: 10.1038/290562a0.
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Repetitive sequences in class-switch recombination regions of immunoglobulin heavy chain genes.免疫球蛋白重链基因类别转换重组区域中的重复序列。
Cell. 1981 Feb;23(2):357-68. doi: 10.1016/0092-8674(81)90131-8.
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Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.产生完整的免疫球蛋白重链基因需要两种类型的体细胞重组。
Nature. 1980 Aug 14;286(5774):676-83. doi: 10.1038/286676a0.
10
Two regulatory elements for immunoglobulin kappa light chain gene expression.免疫球蛋白κ轻链基因表达的两个调控元件。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7041-5. doi: 10.1073/pnas.81.22.7041.