Bergman Y, Rice D, Grosschedl R, Baltimore D
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7041-5. doi: 10.1073/pnas.81.22.7041.
By using internal deletions within a rearranged immunoglobulin kappa light chain gene, the presence of an intron regulatory sequence (enhancer) has been confirmed. Its presence is required for high-level transcription from a plasmid after transfection into myeloma cells. Transfection efficiency was monitored by the activity of a deleted H4 histone gene included in the plasmid. The intron element could be moved upstream of the gene in both orientations, fulfilling the definition of an enhancer. By using 5' deletions, a second regulatory element was located upstream of the "TATA" box, between positions -69 and -104. These two elements both are required for efficient kappa chain gene expression.
通过在重排的免疫球蛋白κ轻链基因内使用内部缺失，已证实存在内含子调控序列（增强子）。将其转染到骨髓瘤细胞后，其存在是从质粒进行高水平转录所必需的。通过质粒中包含的缺失H4组蛋白基因的活性监测转染效率。内含子元件可以以两种方向移动到基因上游，符合增强子的定义。通过使用5'缺失，在“TATA”框上游-69至-104位之间定位了第二个调控元件。这两个元件都是κ链基因高效表达所必需的。
