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Mi-Lnc70调节小鼠胰腺β细胞系的进展并影响胰岛素和胰高血糖素的合成。

Mi-Lnc70 Regulates the Progression of Murine Pancreatic β-Cell Line and Affects the Synthesis of Insulin and Glucagon.

作者信息

Yuan Wen, Sun Dongxue, Wang Jing, Yue Yongli, Li Xueling

机构信息

State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot, 010021, People's Republic of China.

出版信息

Onco Targets Ther. 2025 Sep 3;18:967-978. doi: 10.2147/OTT.S523599. eCollection 2025.

DOI:10.2147/OTT.S523599
PMID:40927431
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12415100/
Abstract

BACKGROUND

Insulinoma, the most common type of pancreatic endocrine tumor, frequently induces hypoglycemia due to persistent hyperinsulinemia. Although Mi-Lnc70 expression progressively increases during pancreatic maturation in mice, the biological role of Mi-Lnc70 in pancreatic β cells remains elusive.

AIM

This study was designed to investigate the role of LncRNA-Mi-Lnc70 in the mouse pancreatic β-cell line MIN6.

METHODS

We performed quantitative real-time PCR, cell counting kit-8 (CCK-8) assay, flow cytometry, transwell assay, wound healing assay, immunofluorescence staining, and Western blotting.

RESULTS

The expression of Mi-Lnc70 was markedly elevated in mouse pancreatic β-cells (MIN6) compared to normal cells. Knockdown of Mi-Lnc70 markedly suppressed the proliferation, migration, and invasion capabilities of MIN6 cells but induced cell apoptosis and triggered G2/M phase cell cycle arrest. Moreover, Mi-Lnc70 knockdown influenced the expression profiles of pancreas-related lncRNAs and miRNAs and decreased the expression of islet-related genes and reduced the protein synthesis of INSULIN, GLUCAGON, and PDX1.

CONCLUSION

Mi-Lnc70 plays an important role in the proliferation, migration, and endocrine-related gene expression in pancreatic MIN6 cells, particularly in the synthesis of PDX1, INSULIN, and GLUCAGON.

摘要

背景

胰岛素瘤是胰腺内分泌肿瘤最常见的类型，由于持续性高胰岛素血症常诱发低血糖。尽管Mi-Lnc70在小鼠胰腺成熟过程中表达逐渐增加，但其在胰腺β细胞中的生物学作用仍不清楚。

目的

本研究旨在探讨长链非编码RNA-Mi-Lnc70在小鼠胰腺β细胞系MIN6中的作用。

方法

我们进行了定量实时聚合酶链反应、细胞计数试剂盒-8（CCK-8）检测、流式细胞术、Transwell检测、伤口愈合检测、免疫荧光染色和蛋白质印迹法。

结果

与正常细胞相比，Mi-Lnc70在小鼠胰腺β细胞（MIN6）中的表达显著升高。敲低Mi-Lnc70可显著抑制MIN6细胞的增殖、迁移和侵袭能力，但诱导细胞凋亡并引发G2/M期细胞周期阻滞。此外，敲低Mi-Lnc70影响胰腺相关长链非编码RNA和微小RNA的表达谱，并降低胰岛相关基因的表达，减少胰岛素、胰高血糖素和PDX1的蛋白质合成。

结论

Mi-Lnc70在胰腺MIN6细胞的增殖、迁移和内分泌相关基因表达中起重要作用，尤其是在PDX1、胰岛素和胰高血糖素的合成中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/b940eefe7143/OTT-18-967-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/79525a6d6739/OTT-18-967-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/5b95228ee04d/OTT-18-967-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/fd1354bcbe9f/OTT-18-967-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/6abdf59c2d9d/OTT-18-967-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/8f8243abd0b1/OTT-18-967-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/b940eefe7143/OTT-18-967-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/79525a6d6739/OTT-18-967-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/5b95228ee04d/OTT-18-967-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/fd1354bcbe9f/OTT-18-967-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/6abdf59c2d9d/OTT-18-967-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/8f8243abd0b1/OTT-18-967-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c12d/12415100/b940eefe7143/OTT-18-967-g0006.jpg

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本文引用的文献

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